摘要
目的:建立大鼠股骨微螺钉种植体周围炎模型,检测其外周血中Foxp3^+调节性T细胞水平的变化。方法:12只雄性SD大鼠分为正常组、炎症组、空白组(n=4),正常组和炎症组单侧股骨远心端植入微螺钉种植体1枚,炎症组在植入种植体后用丝线环扎种植体颈部并使种植体上端暴露于体表外;正常组在植入种植体后严密缝合,植体不外露;空白组仅进行麻醉、切开及缝合等对照处理,未植入种植体。术后第4周取样、采血,拍摄X线片并采用流式细胞术检测外周血中Foxp3^+调节性T细胞(CD4^+CD25^+Foxp3^+)在T淋巴细胞(CD4^+)中的比例。结果:炎症组大鼠种植体周围出现炎性肉芽组织,X线片显示植体颈部周围皮质骨有角形吸收,其外周血中Foxp3^+调节性T细胞占CD4^+T淋巴细胞的比例为(0.66±0.32)%,显著低于正常组(2.69±1.19)%和空白组(4.06±1.34)%,差异有统计学意义(P<0.05);正常组和对照组之间差异无统计学意义。结论:大鼠股骨远心端微螺钉种植体周围炎模型造模成功;该动物模型外周血中Foxp3^+调节性T细胞在数量上存在异常,可能与种植体周围炎的发生有关。
Objective:To establish a peri-microscrew implantitis model in the femur of rats,and investigate the change of proportion of Foxp3^+regulatory T cells in peripheral blood.Methods:Twelve male SD rats were divided into normal group,peri-implantitis group,and blank group(n=4).The normal group and peri-implantitis group accepted an implantation of microscrew implant in the distal end of the femur,while the later one underwent cervical ligation immediately around the implants whose upper part was exposed in vitro.The normal group had no events after the implantation,and the blank group only accepted a“fake”operation without implantation.Four weeks after the operation,the abdominal blood was collected and the femur of each group was taken an X-ray examination and detected the the ratio of Foxp3^+regulatory T cells(CD4^+CD25^+Foxp3^+)in T lymphocytes(CD4^+)using flow cytometry.Results:The peri-implantitis group showed an inflammatory granulation tissue around the implants,whose X-ray film indicated an angular absorption of cortical bone and had a lower proportion(0.66±0.32)%of Foxp3+regulatory T cells in the peripheral blood,significantly lower than normal group(2.69±1.19)%and blank group(4.06±1.34)%(P<0.05).There was no statistically significant difference between normal group and the control group.Conclusion:The model of peri-microscrew implantitis in rats’distal end of femur can be established.Rats with peri-microscrew implantitis have a decrease in the number of Foxp3^+regulatory T cells in peripheral blood,which may be associated with the pathogenisis of peri-microscrew implantitis.
作者
李娜
林崇翔
丁熙
邵侠
赵瑜
屠呈威
LI Na;LIN Chong-xiang;DING Xi;SHAO Xia;ZHAO Yu;TU Cheng-wei(Department of Endodontics,the Affiliated Stomatological Hospital,Wenzhou Medical University,Wenzhou 325000,China;Department of Oral-maxillofacial Surgery,the First Affiliated Hospital,Wenzhou Medical University,Wenzhou 325000,China)
出处
《口腔医学研究》
CAS
北大核心
2018年第7期726-729,共4页
Journal of Oral Science Research
基金
温州市科技计划项目(编号:Y20140325)
温州市科技计划项目(编号:Y20170762)