纯镁阳极氧化膜层不同后处理的细胞相容性

Cytocompatibility of Pure Magnesium Anodic Oxidation Film after Different Post-treatments

目的:通过对纯镁阳极氧化膜层表面进行不同后处理,期望提高复合膜层的耐腐蚀性及细胞相容性。方法:实验分为纯镁阳极氧化处理(A组)、纯镁阳极氧化-硅烷处理(B组)、纯镁阳极氧化-硅烷-植酸处理(C组)、纯镁阳极氧化-硅烷-植酸载铜处理(D组)。采用扫描电镜法研究膜层形貌,激光共聚焦法观察细胞骨架及铺展情况,CCK-8法检测不同膜层表面的细胞粘附、增殖能力。结果:通过对纯镁阳极氧化膜层表面进行硅烷、植酸、载铜处理,有效地减少了其表面的孔隙,其中D组﹥C组﹥B组﹥A组;各膜层表面细胞铺展、粘附、增殖情况皆为C组﹥D组﹥B组﹥A组。结论:纯镁阳极氧化-硅烷-植酸载铜膜层封孔最好,细胞活性较好,硅烷-植酸处理组膜层细胞生物活性最佳。

Objective:To test the corrosion resistance and cell compatibility of pure magnesium anodic oxidation film after different treatments.Methods:The experiments were divided into pure magnesium anodic oxidation group(group A),pure magnesium anodic oxidation-silane treatment group(group B),pure magnesium anodic oxidation-silane-phytic acid treatment group(group C),pure magnesium anodic oxidation-silane-phytic acid coated copper treatment group(group D).Scanning electron microscopy,laser confocal microscopy,and CCK-8 were used to study the morphology of the films,the cytoskeleton and spreading,and the cell adhesion and proliferation ability of different membranes.Results:The pores of the surface of pure magnesium anodized film were effectively reduced by silane,phytic acid and copper loading,among which,group D>group C>group B>group A.The results of the film surface spreading,adhesion,proliferation of four groups were group C>group D>group B>group A.Conclusion:Pure magnesium anodic oxidation-silane-phytic acid coated copper film has the best sealing ability and good cell activity,and the silane-phytic acid treatment group has the best biological activity.