氯吡格雷对小鼠小肠黏膜的损伤及其机制研究

Intestinal mucosal injury induced by Clopidogrel in mice and its mechanism

目的初探氯吡格雷对小鼠小肠黏膜的损伤及可能的机制。方法 20只无特定病原体(SPF)级BALB/c雄性小鼠,随机分为2组:空白对照组、氯吡格雷组,空白对照组灌服生理盐水,氯吡格雷组灌服氯吡格雷,2周后处死小鼠,观察小鼠小肠黏膜大体变化及组织学变化,免疫组织化学(简称免疫组化)法检测小肠黏膜组织中Occludin蛋白的表达情况。结果氯吡格雷组小鼠小肠黏膜大体损伤、组织病理学损伤高于空白对照组(P<0.05);免疫组化测得氯吡格雷组小鼠小肠黏膜Occludin蛋白表达低于空白对照组(P<0.05)。结论氯吡格雷可造成小鼠小肠黏膜损伤;可能通过降低Occludin蛋白表达造成小肠黏膜损伤。

Objective To investigate intestinal mucosal injury induced by Clopidogrel in mice and potential molecular mechanism.Method Twenty SPF grade BALB/C male mice were randomly divided into control group and Clopidogrel group.Mice in Clopidogrel group were treated with Clopidogrel while mice in control group was fed with normal saline.Mice were sacrificed after 2 weeks.Macroscopic and histological changes of the small intestinal mucosa in the two groups were recorded.Expression of Occludin in small intestinal mucosa was determined by immunohistochemistry.Results The macroscopic damage and the histopathologic damage of small intestinal mucosa in Clopidogrel group were significantly higher than those in control group(P<0.05).The expression of Occludin protein in small intestinal mucosa was decreased significantly with treatment of Clopidogrel compared with control group(P<0.05).Conclusions Clopidogrel induces small intestinal mucosa damage through reduction of Occludin expression and inhibition of the repair of small intestinal mucosal injury.