UASB处理模拟酸性矿山废水后的污泥菌种分析

Analysis of sludge strains after UASB treatment of simulated acid mine wastewater

以呼和浩特市章盖营污水处理厂二沉池回流污泥为接种污泥,用模拟酸性矿山废水驯化后获得含硫酸盐还原菌(Sulfate-Reducing Bacterium,SRB)的厌氧污泥,将Cu2+、Zn2+、Ni2+和Cr6+四种重金属离子设定为UASB的主要处理污染物。在UASB装置底部侧管用离心管取污泥样品少许,经过离心,固定等前处理程序之后,利用扫描电镜观察并拍照、沉积污泥XRD分析和PCR-DGGE及测序分析方法对污泥菌种进行分析。实验结果表明,活性污泥中SRB呈杆状和球状,菌体外部大部分被白色沉淀覆盖;HRT 31 h出水p H 5时,群落的生物多样性高于活性恢复后状态;发酵产酸菌得以大量富集,UASB反应器处理效果恢复后,发酵产酸菌优势度降低,而脱硫弧菌属5,7(Desulfovibrio sp.)得以大量富集。

The recycling sludge in secondary sedimentation tank from Zhanggaiying(Huhhot)wastewater treatment plant was used as cultivated sludge to foster anaerobic sludge containing sulfate reducing bacteria(SRB)in a UASB reactor.Then the effect of heavy metal ions loading(Cu2+,Zn2+,Ni2+and Cr6+)was investigated.At the bottom of the UASB device,getting a small number of sludge samples w from the centrifuge tube,after centrifugation,fixation and other pre-treatment procedures,The sludge strains were analyzed by SEM,XRD analysis,PCR-DGGE analysis and sequencing analysis.Results showed that In activated sludge,SRB is a rod and ball,and most of the outside of the bacteria is covered by white precipitation.When the pH was 5 and HRT was 31h,the biodiversity of the community was higher than that after the restoration of activity.The acid bacteria produced by fermentation were enriched in large amounts.After the treatment effect of the UASB reactor was restored,the advantage of the acid bacteria produced by fermentation was reduced,while the Desulfovibrio sp.was enriched in large amounts.