番茄褪绿病毒在湖南省首次发生

First Report of the Occurance of Tomato chlorosis virus in Hunan Province

2016年7月在湖南省蔬菜病害调查中发现,4种茄科蔬菜表现出叶脉间褪绿、叶片黄化等症状,疑似被番茄褪绿病毒(Tomato chlorosis virus,To CV)感染,同时叶片背面聚集了大量烟粉虱。采用To CV热激蛋白(heat shock protein 70,HSP70)序列的特异性引物对采集的番茄、茄子、辣椒、马铃薯样品和烟粉虱样品进行RT-PCR检测,均扩增出目标条带,且扩增序列与北京番茄To CV分离物(KC887999.1)部分序列相似度为99.0%,确认采集样品被To CV感染。4种茄科蔬菜To CV的感染率达70%~100%;发病叶片上烟粉虱的带毒率为66.7%~87.5%;鉴定出烟粉虱的生物类型为MED烟粉虱。这是湖南省首次确认该病毒,需要引起关注和加强防范。

During the investigation for vegetable diseases of Hunan Province in July 2016,symptoms of intervein chlorosis and leaf yellowing were discovered on Solanaceae vegetable leaves,including tomato,pepper,eggplant and potato plants.The symptoms were suspected as Tomato chlorosis virus(ToCV)and a large number of Bemisia tabaci were found on the back of leaves.The infected leaves of tomato,pepper,eggplant and potato and Bemisia tabaci samples were detected by RT-PCR using gene specific primers of ToCV heat shock protein 70(HSP70)sequence,and an expected fragment were obtained.The results of sequence analysis revealed that the fragment shared the highest nucleotide identity at 99.0%with the isolates from Beijing(KC887999.1).The infection rate of ToCV of these 4 kinds of Solanaceae vegetables was 70%-100%.Viruliferous rate of Bemisia tabaci was 66.7%-87.5%.The whitefly biotype was MED.This is the first time that Hunan Province confirms the existing of ToCV,which needs special attention and stronger prevention.