经小脑延髓移植骨髓间充质干细胞对受损脊髓白质的影响

Effects of bone marrow stem cells transplanted via cisterna magna on injured spinal cord white matter

目的观察经小脑延髓移植骨髓间充质干细胞(BMSCs)对受损脊髓白质的修复作用。方法改良Allen法构建大鼠脊髓损伤(SCI)模型,随机分成模型组和BMSCs组。BMSCs组给予10μL BMSCs悬液,模型组给予等量生理盐水。BBB评分评估术后1、7、14、21、28 d神经功能恢复情况,4.7 T核磁扫描仪研究大鼠脊髓白质变化;HE染色观察SCI后的病理变化;荧光显微镜检测Hoechst 33342标记的BMSCs数目。结果 SCI术后7、14、21、28 d BMSCs组BBB评分均显著高于模型组(P<0.05),BMSCs组脊髓白质纵向弥散梯度接近于模型组(P>0.05)。BMSCs组脊髓病灶中心平均面积明显小于模型组(P<0.05)。HE染色显示BMSCs组14、28 d脊髓损伤的恢复情况明显优于模型组。BMSCs组术后7 d Hoechst 33342标记的细胞信号水平高于模型组,随后从7 d到1个月逐渐降低,差异有高度统计学意义(P<0.01)。结论经小脑延髓移植BMSCs对受损脊髓白质的恢复有着积极的促进作用,这为SCI的治疗提供了一种新途径。

Objective To investigate the effect of bone marrow stem cells(BMSCs)transplanted via cisterna magna on the rehabilitation of damaged spinal cord white matter.Methods The spinal cord injury(SCI)model was carried out by a modified Allen method,assigned into the control group and BMSCs group randomly.The BMSCs group and control group were given 10μL BMSCs suspension and 10μL physiological saline via cistern magna respectively.BBB scale was utilized to estimate the neurological recovery on 1,7,14,21,28 d postoperatively after SCI.The white matter changes in the injured spinal cord were tested using 4.7 T MRI scanner.HE staining was applied to access the rehabilitation condition.Flourescence microscope was performed to identify the signal level of Hoechst 33342 marked BMSCs in the injured spinal cord.Results The BBB scores in the BMSCs group were significantly higher than that of the control group on 7,14,21,28 d after SCI(P<0.05).There was no statistically significant difference between the two groups regard to the longitudinal diffusion gradients of the damaged spinal cord white matter(P>0.05).The average size of the lesion center in the BMSCs group was significantly smaller than that of the control group(P<0.05).HE staining revealed that the recovery of the BMSCs group was better than the control group at 14,28 d after SCI.The counts of Hoechst 33342 labelled cells were less in the control group than that in the BMSCs group at 7 d.Subsequently,they continued to decrease from 7 d to 4 weeks later,with highly statistically significant difference(P<0.01).Conclusion Bone marrow mesenchymal stem cells transplanted via cisterna magna has a positive role in promoting the rehabilitation of damaged spinal cord white matter,which is a novel way of treating SCI.