裸花紫珠提取物对银屑病作用及机制研究

Study of effect and potential mechanism of Callicarpa nudiflora extract against psoriasis

目的探讨裸花紫珠提取物抗银屑病作用。方法 60只KM小鼠按照随机数字表法分为模型组、裸花紫珠提取物低剂量组、裸花紫珠提取物中剂量组、裸花紫珠提取物高剂量组和阳性药对照组,每组各12只。分别灌胃给予生理盐水,0.6、1.2、2.4 g/kg裸花紫珠提取物,1 mg/kg甲氨蝶呤,连续7 d。HE法检测完整颗粒层鳞片数;Western blot技术检测Caspase3、Cleaved-Caspase3、Bax、BCL-2蛋白表达;Real-time PCR技术检测Caspase3、Bax、BCL-2基因m RNA表达。结果裸花紫珠中、高剂量组鼠尾完整颗粒层鳞片数明显高于模型组(P<0.05);裸花紫珠中、高剂量组促凋亡蛋白Caspase3、Cleaved-Caspase3、Bax蛋白和m RNA表达均高于模型组(P<0.05),抗凋亡蛋白BCL-2蛋白和m RNA表达下调(P<0.05)。结论裸花紫珠提取物通过诱导凋亡发挥抗银屑病作用。

Objective To determine the anti-psoriasis effect of Callicarpa nudiflora extract.Methods Sixty KM mouses were randomly divided into 5 groups by random number table(n=12),including model group,Callicarpa nudiflora low-dose group,Callicarpa nudiflora middle-dose group,Callicarpa nudiflora high-dose group and positive control group.They were given to normal saline,Callicarpa nudiflora extract(0.6 g/kg or 1.2 g/kg or 2.4 g/kg)and 1 mg/kg methotrexate by gastric perfusion for seven days respectively.Lateral line scales of granular layer were detected by HE method.The expression of Caspase3,Cleaved-Caspase3,Bax and BCL-2 mRNA and protein were detected by Real-time PCR and Western blot.Results Lateral line scales of granular layer of Callicarpa nudiflora middle-dose and high-dose groups was increased than those in the model group(P<0.05).The expression of the pro-apoptotic proteins Caspase3,Cleaved-Caspase3,Bax mRNA and protein in callicarpa nudiflora middle-dose and high-dose groups were up-regulated than those in the model group(P<0.05),the expression of the anti-apoptotic protein BCL-2 mRNA and protein was down-regulated(P<0.05).Conclusion The Callicarpa nudiflora extract can exert anti-psoriatic effect by inducing apoptosis.