合浦珠母贝转录因子PF-CREB3L2基因的克隆和鉴定

Cloning and mineralization-related functions of the PFCREB3L2 gene in Pinctada fucata

克隆得到了合浦珠母贝(Pinctada fucata)PF-CREB3L2蛋白的c DNA序列,其c DNA全长1 983 bp,其中开放阅读框长度为1 728 bp,编码的蛋白含有575个氨基酸残基。组织表达分布实验发现,其在合浦珠母贝内脏囊中表达量最高,在外套膜和鳃中也有大量表达,推测其广泛参与合浦珠母贝的贝壳形成等生理活动。贝壳损伤修复实验中发现,在合浦珠母贝贝壳受到损伤后,PF-CREB3L2和基质蛋白的表达量会迅速上升,且PF-CREB3L2的峰值出现更早,推测其通过影响基质蛋白转录,参与合浦珠母贝生物矿化的调控过程。PF-CREB3L2与合浦珠母贝基质蛋白表达相关性的分析发现,PF-CREB3L2与Prisilkin39、KRMP的表达量呈现显著的正相关,说明其可能特异性地调控某些基质蛋白的转录。

In this study,the cDNA sequence of PF-CREB3L2 protein was cloned by 1 983 bp,the length of the open reading frame was 1 728 bp,and the encoded protein comprised 575 amino acid residues.The tissue expression distribution revealed that it was most expressed in the viscera group.In addition,it was expressed in the mantle and gills.We anticipated that it was widely involved in the physiological activities,including the shell formation.In the shell damage repair experiment,the expression of PF-CREB3L2 and matrix protein increased rapidly,and the peak of PF-CREB3L2 appeared earlier,suggesting its involvement in the regulation of biomineralization of Pinctada fucata by affecting the transcription of matrix protein.The regulation process in biomineralization between PF-CREB3L2 and the matrix protein revealed a significant positive correlation between PF-CREB3L2 and Prisilkin39 and KRMP expression,suggesting that it could specifically regulate the transcription of specific matrix proteins.