支气管哮喘急性发作期患者血小板线粒体功能变化及其与氧化应激的相关性研究

Platelet Mitochondrial Functional Change and Its Correlation with Oxidative Stress in Patients with Acute Attack of Bronchial Asthma

目的探讨支气管哮喘急性发作期患者血小板线粒体功能变化及其与氧化应激的相关性。方法选取2016年4月—2017年5月新疆维吾尔自治区中医医院呼吸科门诊及住院的支气管哮喘急性发作期患者122例作为哮喘组,另选取同期体检健康者44例作为对照组。比较两组受试者血小板线粒体膜电位、三磷腺苷(ATP)、细胞色素C氧化酶(COX)活性及血清8-异前列腺素(8-iso)、硫代巴比妥酸反应物(TBARS)、过氧化脂质(LPO)水平;支气管哮喘急性发作期患者血小板线粒体功能指标与氧化应激指标的相关性分析采用Pearson相关分析。结果哮喘组患者血小板线粒体膜电位、ATP及COX活性低于对照组(P<0.05)。哮喘组患者血清8-iso、TBARS、LPO水平高于对照组(P<0.05)。Pearson相关分析结果显示,支气管哮喘急性发作期患者血小板线粒体膜电位与血清LPO水平呈负相关(r=-0.198,P<0.05),血小板线粒体ATP与血清8-iso、TBARS水平呈负相关(r值分别为-0.193、-0.203,P<0.05),血小板线粒体COX活性与血清8-iso、LPO水平呈负相关(r值均为-0.423,P<0.05)。结论支气管哮喘急性发作期患者存在血小板线粒体功能损伤,主要表现为呼吸链抑制,且血小板线粒体功能损伤与氧化应激有关,这对探究支气管哮喘的发病机制具有一定参考价值。

Objective To investigate the platelet mitochondrial functional change and its correlation with oxidative stress in patients with acute attack of bronchial asthma.Methods From April 2016 to May 2017 in the Department of Respiratory Medicine,the Traditional Chinese Medicine Hostital of Xinjiang Uygur Autonomous Region,a total of 122 outpatients and inpatients with acute attack of bronchial asthma were selected as case group,meanwhile a total of 44 healthy volunteers admitted to this hospital for physical examination were selected as control group.Membrane potential,ATP and cytochrome C oxidase(COX)activity of platelet mitochondria,serum levels of 8-isoprostane(8-iso),thiobarbituric acid reactive substance(TBARS)and lipid peroxidase(LPO)were compared between the two groups;Pearson correlation analysis was used to analyze the correlations of index of platelet mitochondrial function with index of oxidative stress in patients with acute attack of bronchial asthma.Results Membrane potential,ATP and COX activity of platelet mitochondria in case group were statistically significantly lower than those in control group(P<0.05).Serum levels of 8-iso,TBARS and LPO in case group were statistically significantly higher than those in control group(P<0.05).Pearson correlation analysis results showed that,membrane potential of platelet mitochondria was negatively correlated with serum LPO level in patients with acute attack of bronchial asthma(r=-0.198,P<0.05),ATP of platelet mitochondria was negatively correlated with serum levels of 8-iso(r=-0.193)and TBARS(r=-0.203),respectively(P<0.05),COX activity of platelet mitochondria was negatively correlated with serum 8-iso(r=-0.423)and LPO(r=-0.423)level,respectively(P<0.05).Conclusion Platelet mitochondrial function is damaged in patients with acute attack of bronchial asthma,mainly performed as respiratory chain inhibition,meanwhile platelet mitochondrial functional is closely correlated with oxidative stress,which is a valuable reference for study on pathogenesis of bronchial asthma.