HHIP基因多态性与新疆蒙古族慢性阻塞性肺疾病易感性的关系研究

Relationship between HHIP Gene Polymorphism and Chronic Obstructive Pulmonary Disease Susceptibility in Mongolian Population in Xinjiang,China

目的探讨HHIP基因多态性与新疆蒙古族慢性阻塞性肺疾病(COPD)易感性之间的关系。方法选取2016年1月—2017年6月新疆巴音郭楞蒙古自治州医院和新疆博尔塔拉蒙古自治州医院诊断为COPD的蒙古族患者259例(病例组),选取同期体检中心的体检健康的蒙古族志愿者245例(对照组)。测定研究对象的第1秒用力呼气末容积占预计值百分比(FEV_1%)、用力肺活量(FVC)及FEV_1/FVC。提取患者外周血DNA,运用SNaPshot分型技术检测HHIP基因rs1489758、rs1492820位点多态性。结果病例组与对照组HHIP基因rs1489758、rs1492820位点基因型、等位基因分布比较,差异有统计学意义(P<0.05)。病例组HHIP基因rs1489758、rs1492820位点不同基因型患者的FEV_1%比较,差异有统计学意义(P<0.05)。HHIP基因rs1489758位点基因型GT[OR=0.582,95%CI(0.396,0.854)]、等位基因T[OR=0.639,95%CI(0.463,0.884)]和rs1492820位点基因型GG[OR=1.975,95%CI(1.153,3.384)]、等位基因G[OR=1.388,95%CI(1.076,1.790)]是发生COPD的影响因素(P<0.05)。结论 HHIP基因rs1489758、rs1492820位点多态性可能与新疆蒙古族人群COPD的发生有关。

Objective To explore the relationship of single nucleotide polymorphism of HHIP gene with chronic obstructive pulmonary disease(COPD)susceptibility in Mongolian population in Xinjiang,China.Methods The participants enrolled were all from Bayingol Mongolian Autonomous Prefecture Hospital and Bortala Mongolian Autonomous Prefecture Hospital,including 259 inpatients and outpatients(case group)diagnosed as COPD,and 245 healthy controls(control group)underwent physical examination in physical examination center,from January 2016 to June 2017.Pulmonary function parameters such as FEV1%,FVC,and FEV1/FVC were measured.The SNaPshot technique for SNP genotyping was used to detect the polymorphisms(rs1489758,rs1492820)of HHIP gene in peripheral venous blood cells.Results The patterns of genotype and allele frequencies of rs1489758 and rs1492820 at the HHIP gene differed significantly between the groups(P<0.05).FEV 1%predicted varied substantially between the patients in the case group by rs1489758 and rs1492820 genotypes(P<0.05).The factors associated with COPD are genotype GT〔OR=0.582,95%CI(0.396,0.854)〕,allele T〔OR=0.639,95%CI(0.463,0.884)〕of rs1489758 and genotype GG〔OR=1.975,95%CI(1.153,3.384)〕,allele G〔OR=1.388,95%CI(1.076,1.790)〕of rs1492820(P<0.05).Conclusion The two single nucleotide polymorphisms(rs1489758 and rs1492820)at the HHIP gene locus significantly correlate with COPD susceptibility in Mongolian population in Xinjiang,China.