利用Illumina MiSeq高通量测序技术分析原料乳的菌群分布

Application of Illumina MiSeq High-Throughput Sequencing Technique to Analyze Bacterial Distribution in Raw Milk

为测定原料乳中的菌群分布情况,应用Illumina MiSeq高通量测序方法,主要对14个奶牛场的原料乳进行采集,通过DNA测序确定菌群的多样性及致病菌的分布情况,进行操作分类单元聚类分析、Alpha多样性分析、物种分类分析3方面的测定。结果表明不同牧场的菌群存在多样性,位于前列的分别是肠球菌属(Enterococcus)、芽孢杆菌属(Bacillus)、不动杆菌属(Acinetobacter)、乳球菌属(Lactococcus),同时表明,个别牛场原料乳中有金黄色葡萄球菌(Staphylococcus aureus)和志贺菌(Shigella)的存在。

This study was designed to determine the distribution of bacterial populations in raw milk by Illumina MiSeq high-throughput sequencing method.The bacterial diversity and the distribution of pathogenic bacteria in raw milk samples collected from 14 dairy farms were determined by means of DNA sequencing with operational taxonomic unit(OTU)clustering analysis,Alpha diversity analysis and species classification analysis.The results showed that bacterial diversity was found in the milk samples from different farms with the genera Enterococcus,Bacillus,Acinetobacter and Lactococcus being the most diverse.At the same time,Staphylococcus aureus and Shigella were detected in only a few milk samples.