冷藏过程中三疣梭子蟹的营养物质变化

Changes in Nutrients of Portunus trituberculatus during Chilled Storage

为了解冷藏条件下三疣梭子蟹的营养物质变化规律,采用基于核磁共振(nuclear magnetic resonance,NMR)的代谢组学技术对冷藏过程中的梭子蟹肌肉和肝胰腺的营养物质进行分析。结果表明,NMR技术共检测到梭子蟹肌肉中的30种物质和肝胰腺中的27种物质。鲜活梭子蟹在4℃海水中冷藏保存,肌肉的营养物质变化主要发生在前2 d,而肝胰腺的营养物质变化主要发生在第1天。冷藏可导致肌肉谷氨酸、谷氨酰胺、精氨酸、牛磺酸和三磷酸腺苷水平的显著降低,乳酸、三甲胺-N-氧化物、肌苷、肌苷酸和单磷酸腺苷水平的显著升高;同时导致肝胰腺中多种氨基酸、甜菜碱、牛磺酸、葡萄糖、三甲胺-N-氧化物、胆碱-O-硫酸和2-吡啶甲醇等水平的显著降低,而乳酸水平显著升高。这些物质的变化显著改变了梭子蟹的口味和营养价值。

The changes of nutrients of the muscle and hepatopancreas of Portunus trituberculatus during chilled storage were analyzed using nuclear magnetic resonance(NMR)-based metabolomics.The results showed that a total of 30 nutrients were detected in swimming crab muscle and 27 nutrients in hepatopancreas.The levels of muscle nutrients changed mainly during the first two days of chilled storage;glutamate,glutamine,arginine,taurine,and ATP significantly increased while lactic acid,trimethylamine-N-oxide,inosine,inosine monophosphate,and adenosine monophosphate significantly decreased.The levels of hepatopancreas nutrients mainly changed during the first day of storage;the levels of the hepatopancreas amino acids,betaine,taurine,glucose,trimethylamine-N-oxide,choline-O-sulfate,and 2-pyridinemethanol significantly decrease while lactic acid significantly increased.As a result of these changes the taste and nutritional value of swimming crabs changed significantly.