阀切换离子色谱技术同时检测饮料中的糖类、甜味剂和防腐剂

Simultaneous Determination of Sugars, Edulcorants, and Preservatives in Beverages Using Ion Chromatography Coupled with Valve Switching

建立阀切换离子色谱技术同时检测饮料中的糖类、甜味剂和防腐剂的方法。样品经简单前处理进样于IonPac~■ AS11-HC(4 mm×250 mm)阴离子分离柱,通过设置合适的阀切换时间,在AS11-HC柱上保留较弱的糖类物质先被碱性淋洗液洗出,收集在定量环中。而强保留的防腐剂随着淋洗液逐渐洗出,紫外检测器检测。收集在定量环中的糖类物质再经Na OH淋洗液冲洗进入CarboPac~■PA10(4 mm×250 mm)柱中分离,进行电化学检测。实验结果表明,在0.5~2.5 mg/L的线性范围内,方法的回归系数r^2不小于0.999 0,重现性相对标准偏差不大于6.72%,回收率为80.0%~120.0%。该方法可广泛应用于饮料行业中的生产工艺质量控制或最终成品的成分检测。

An ion chromatography method for the determination of three sugars,four preservatives and one edulcorant in beverages using valve switching technique was established.After simple pretreatment,the sample was injected into an IonPac^?AS11-HC(4 mm×250 mm)separation column.By setting an appropriate valve switching time,the carbohydrates with weak retention in the AS11-HC column were first washed out and collected in a quantitative loop.The preservatives retained strongly were gradually washed out by the eluent and detected by a UV detector.The carbohydrates collected were then rinsed into a CarboPac^?PA10(4 mm×250 mm)column and separated using NaOH eluent for electrochemical detection.The experimental results showed that the regression coefficients(r 2)of the calibration curves were more than 0.999 0 in the range of 0.5–2.5 mg/L.The reproducibility expressed as relative standard derivation(RSD)was less than 6.72%,and the recovery ranged between 80.0%and 120.0%.The method can be widely applied to quality control or the detection of components in final products in the beverage industry.