顺铂靶向食管癌细胞周期Cx43表达的研究

A Study on Cisplatin Targeting Cx43 Expression in Cell Cycle of Esophageal Cancer

目的:为研究顺铂治疗食管鳞癌细胞的靶向作用。方法:本研究使用流式细胞技术双变量分析检测顺铂对食管癌细胞周期进程和癌细胞周期的连接蛋白43(connexin 43,Cx43)表达的影响。结果:顺铂对食管鳞癌细胞周期的影响主要作用于S期的DNA复制,细胞阻滞于S期,G2/M期减少。顺铂诱导食管鳞癌细胞周期S和G2/M期的Cx43表达的大幅度改变。低浓度顺铂(由0~2μmol/L),Cx43表达增强;顺铂渐高浓度(2~12μmol/L),细胞Cx43表达由强逐渐变弱,特别是G2/M期细胞的Cx43表达活跃,易受顺铂影响。结论:我们的研究表明以顺铂处理食管鳞癌细胞,癌细胞周期的S期和G2/M期的Cx43表达与S期的DNA复制一样可作为的潜在治疗靶标。顺铂靶向作用细胞周期S和/或G2/M期细胞的特性可能减少或避免对非分裂细胞的影响。

Objective:To study the target effect of cisplatin on treating esophageal squamous cell carcinomas(ESCC).Methods:In this study,the influence of cisplatin on cell cycle progression of esophageal cancer and connexin 43(Cx43)expression in cancer cell cycle were detected by bivariate flow cytometric analysis.Results:The effect of cisplatin on cell cycle of ESCC was mainly due to DNA replication of S phase cells,arrest of cells in the S phase,and the G2/M phase cells decreases after drug exposure.Cx43 expression in S and G2/M phase of the cell cycle for cisplatin-treated ESCC was significantly altered.Cx43 expression was enhanced in low concentration of cisplatin(from 0 to 2μmol/L).In high concentration of cisplatin(from 2 to 12μmol/L),cellular Cx43 expression was gradually weakened.In particular,Cx43 expression of G2/M phase cells was more active and susceptible to cisplatin.Conclusions:Our study shows that Cisplatin serves as potential therapeutic target for Cx43 expression of the S and G2/M phase of cancer cell cycle as well as DNA replication of the S phase during cisplatin treating ESCC.The characteristics of cisplatin targeting S and/or G2/M phase of the cell cycle may reduce or avoid the effects of nondividing cells.