低聚肽和VC复合固体饮料的安全性毒理学评价

Toxicological and Safety Assessment of Oligopeptides and Vitamin C Compound in Solid Drink

为了评价低聚肽和维生素C复合固体饮料作为功能性食品食用的毒理学安全性,根据我国卫生部《保健食品检验与评价技术规范》(2003)进行安全性毒理学研究。研究结果表明:低聚肽和维生素C复合固体饮料对小鼠急性经口最大耐受剂量大于20 g/kg BW,根据急性毒性分级标准规定,属无毒级;遗传毒性试验(Ames试验、小鼠骨髓细胞微核试验、小鼠精子畸形试验)结果为阴性;30天喂养试验表明,按人体推荐量的25、50和100倍即2.1 g/kg、4.2 g/kg、8.3 g/kg为低、中、高剂量时,各剂量组动物生长发育良好,大鼠体重、增重、食物利用率、脏器重量、脏/体比、血液学指标及血生化指标与对照组比较均无显著性差异(P>0.05);大体解剖和组织病理学检查未见与样品有关的异常改变。通过以上的试验结果可以判定低聚肽和维生素C复合固体饮料具有较好的食用安全性。

In order to evaluate the toxicological safety of oligopeptides and vitamin C compound in solid drink.Toxicological and safety assessments were conducted,according to the Technical Standards for Testing and Assessment of Health Food by Healthy Ministry 2003,the results showed that the maximum tolerated dose of oligopeptides and vitamin C compound in solid drink on mice was more than 20 g/kg.BW.Therefore,the sample fell into the scale of non-toxicity.The genotoxicity tests including Ames test,bone marrow cell micronucleus,sperm shape abnormality were all negative.The results of 30-days test showed that the animals of control group and each dose group were well developed,without death and abnormal clinical sign.The body weight,food intake food utilization rate,organ weight,organ/body ratios,hematological parameters and blood biochemical indexes had no significant difference(P>0.05)between the control group and the treatment groups.There are no abnormal pathological changes in liver,kidney,stomach,intestine,spleen,testicle and ovary of rats in treatment group.The conclusion is that the oligopeptides and vitamin C compound in solid drink are safe with no evident toxic effect.