氮硫共掺杂碳点荧光探针检测黄芩素的研究

Fluorescence probe of nitrogen and sulfur co-doped carbon dots to detect baicalein

以硫代苹果酸和谷氨酰胺为原料,利用微波合成法制备了氮硫共掺杂碳点(NSCDs),用透射电子显微镜(TEM)、傅里叶变换红外光谱(FT-IR)、紫外-可见吸收光谱(UV-Vis)和荧光光谱考察了NSCDs的性质。NSCDs的荧光强度随着黄芩素浓度的增加而逐渐降低,浓度在2×10-6M^4×10-5M范围内的黄芩素与NSCDs的荧光强度呈良好的线性关系,检出限为7.5×10-8M,以此为基础构建了检测黄芩素的荧光探针。初步探讨了黄芩素与NSCDs相互作用的反应机理。该方法还被成功应用于健康人体尿样和血样中微量黄芩素的测定,回收率在97.0%~103.5%之间。

Carbon and sulfur co-doped carbon dots(NSCDs)were prepared by microwave synthesis method using malic acid and glutamine.The properties of NSCDs were investigated by transmission electron microscopy(TEM),Fourier transform-infrared spectroscopy(FT-IR),ultraviolet–visible spectroscopy(UV-Vis)and fluorescence spectroscopy.The fluorescence intensity of NSCDs decreased gradually with the increase of baicalein concentration.The concentrations of baicalein in the range of 2×10^-6 M^4×10^-5 M showed a good linear relationship with the fluorescence intensity of NSCDs with the detection limit of 7.5×10-8 M.A fluorescence probe for detection of baicalein was constructed based on this.The reaction mechanism between baicalein and NSCDs was discussed.This method has been successfully applied to the detect the baicalein in healthy human urine and blood samples,the recovery rate is between 97%and 103.5%.