生防因子间型假丝酵母C410液体酵母保存研究

Preservation of Liquid Formulation of the Biocontrol Agent Candida intermedia C410

对生防菌间型假丝酵母(Candida intermedia)C410菌株的液体酵母保存方法进行研究。结果表明,当保存起始细胞浓度为2×109个细胞/m L,4℃恒温保存,在磷酸缓冲液PPB中添加5%海藻糖和1 mmol/L维生素C时,能够使C410酵母细胞保持高效活力持续90 d以上。对保存90 d的液体酵母防效进行测定,发现其防治效果与新鲜酵母细胞悬浮液的防效相当,都能抑制番茄灰霉病菌的生长繁殖,保持番茄果实品质完整。

The preservation of liquid formulation of the biocontrol agent Candida intermedia C410 was primarily explored.Results showed that,while the initial preserving concentration was 2×109 cells/mL,storage temperature was 4℃,liquid formulation of C410 with high viability of cells were phosphate buffers supplemented with 5%trehalose and 1 mmol/L ascorbic acid.The liquid formulation kept high viability of yeast cells for more than 90 days.Suspensions of liquid formulation after 90 days and fresh yeast cells were tested against B.cinerea on tomato fruits,both suspensions exhibited prospective biocontrol effect,gray mould of tomato was inhibited while the quality of tomato fruits maintain completely good.