家兔肌肉组织miRNA Solexa测序与生物信息学分析

Solexa Sequencing and Bioinformatics Analysis on miRNA from Rabbit Muscle

miRNA是一类长度约22 bp的小分子非编码RNA,在骨骼肌生长和肌细胞分化过程中起着重要的调控作用。利用Solexa测序技术对3只84日龄成年公兔的腿肌进行了miRNA测序,旨在评估高通量测序在发掘家兔miRNA中的数据质量以及解析家兔肌肉组织中miRNA的生物学特征。结果表明,除前4位碱基的错误率略高于0.5%外,其他位置的碱基错误率均低于0.5%,说明测序质量好、可信度高,获得的序列可用于后续miRNA的鉴定分析。纯净序列中其他类型RNA的比例仅占2.07%。肉兔肌肉中18~24 nt的小RNA首位碱基以U为主,其次为碱基A,具有很强的偏向性。miRNA序列长度主要分布在20~24 nt范围内,占纯净序列的90%以上。miRNA长度分布的不平衡和首位碱基的偏向性可能与miRNA的作用机理和具体生物学功能有关。

miRNA as a kind of micromolecule non-coding RNA with the length of about 22 bp plays an important regulatory role in skeletal muscle growth and muscle cell differentiation.This research uses Solexa sequencing technology to conduct miRNA sequencing on three eight-four-day-old adult male rabbits,aiming at evaluating the quality of high-throughput sequencing in meat-rabbit miRNA data mining,and exploring the biological characteristics of miRNA in meat-rabbit muscular tissues.Results show that,except for bases in first three positions with an error rate slightly higher than 0.5%,bases in other positions have an error rate of lower than 0.5%,which indicates good sequencing quality,high reliability,and the applicability of obtained sequence in subsequent miRNA identification and analysis.Other types of RNA in pure sequences only take up 2.07%.The 18~24 nt small RNA first base in meat-rabbit muscle is mainly U base,and A base for the second with strong directivity.miRNA sequence length is mainly within the range of 20~24 nt,which takes up more than 90%of pure sequences.miRNA sequence length imbalance and the directivity of first-place base may be related to the action mechanism and specific functions of miRNA.