小麦麸皮低聚糖生产中高温预处理条件及其提取工艺优化

HIGH-TEMPERATURE PRETREATMENT CONDITIONS ON WHEAT BRAN OLIGOSACCHARIDE PREPARATION AND THE EXTRACTION PROCESS OPTIMIZATION

为了提高小麦麸皮低聚糖的生产效率,降低其生产成本,采用单因素试验研究了150℃以上不同温度下的麸皮预处理条件和木聚糖酶添加量、酶解p H、酶解时间和酶解温度对小麦麸皮中木聚糖溶出量的影响,并研究了糖液脱色工艺中活性炭添加量、脱色时间和脱色温度对脱色率的影响。150℃以上酸性条件蒸煮的最佳工艺为:乙酸添加量为麸皮干重的0.15%、蒸煮温度160℃、蒸煮时间20 min,在此蒸煮条件下,可溶性戊聚糖得率最高,达到麸皮干重的15.5%,色泽较浅,有害物质糠醛含量较少。木聚糖酶酶解的最佳工艺参数为:木聚糖酶添加量0.20%、酶解p H值5.0、酶解时间4 h、酶解温度45℃,在此酶解条件下,可溶性戊聚糖溶出率为27.70 g/L。活性炭脱色最佳工艺参数为:活性炭添加量6%、脱色温度60℃、脱色时间50 min,在此脱色条件下,糖液脱色率达到97%,脱色过的糖液颜色澄清。优化后的工艺条件成本低、效率高,对小麦麸皮低聚糖的生产应用具有一定的参考作用。

The oligosaccharide extracted from wheat bran have the functions of promoting probiotic proliferation,enhancing immune response,inhibiting oxidative stress induced by high fat diet,moisturizing intestines and relieving defecation,and decreasing blood glucose.In order to improve the production efficiency of wheat bran oligosaccharides,the effect of pretreatment conditions of wheat bran,xylanase addition amount,enzymatic hydrolysis pH,enzymatic hydrolysis time and enzymatic hydrolysis temperature on the xylanolysis amount of wheat bran were studied by single factor experiment at different temperatures above 150℃.The effect of activated carbon amount,decolorization time and decolorization temperature on the decolorization rate of sugar solution were also studied.The results showed that the optimum cooking conditions above 150℃were as follows:acetic acid content 0.15%of dry weight of wheat bran,cooking temperature 160℃and cooking time 20 min.Under these conditions,the yield of soluble pentosan was the highest,reaching 15.5%of dry weight of wheat bran,the color was light and the harmful substance furfural content was also less.The optimum technological parameters of xylanase hydrolysis were as follows:xylanase addition 0.20%,enzymatic hydrolysis pH 5.0,enzymatic hydrolysis time 4 h and enzymatic hydrolysis temperature 45℃,under which the dissolution rate of soluble pentosan was up to 27.70 g/L.The optimum decolorization parameters were as follows:the amount of activated carbon 6%,the decolorization temperature 60℃and the decolorization time 50 min,under which the decolorization rate of sugar solution reached to 97%and the color of afforded decolorized sugar solution was clarified.The optimized technological conditions have high production efficiency but low cost,which can be used as reference for the wheat bran oligosaccharides production.