烟草黄瓜花叶病毒抗性位点发掘

Identification of QTL Associated with Resistance to Cucumber Mosaic Virus in Tobacco

烟草黄瓜花叶病毒(CMV)是烟草生产中主要病害之一,发掘其抗性位点,培育抗病品种是最有效的策略之一。本研究利用高抗CMV烤烟品种台烟8号为轮回亲本,优质感病烤烟品种NC82为供体亲本,配置BC1F1群体。在此基础上,利用组织快繁技术对200份BC1F1群体进行快繁,苗期人工接种CMV,获得稳定、可靠的表型数据。利用SSR标记构建遗传图谱,进而利用单向方差分析和完备复合区间作图法发掘CMV抗性位点。结果表明,单向方差分析法分别检测到5个与发病率性状和病情指数性状相关的标记;完备复合区间法共定位到5个QTL,其中2个与发病率性状相关,3个与病情指数性状相关。相关定位结果对开展分子育种辅助改良烟草CMV抗性具有一定意义。

Cucumber mosaic virus(CMV)is one of the most serious diseases affecting tobacco(Nicotiana tabacum L.).It is an effective way to prevent CMV by mining,identification and utilization of resistant genes.In this study,a BC1F1 population was constructed from a cross between recurrent parent Taiyan8 and donor parent NC82.Based on this population,50 plants generated from each BC1F1 individual using tissue culture method were evaluated for resistance to CMV.Furthermore,QTLs associated with resistance to CMV were identified by one-way ANOVA and Inclusive Composite Interval Mapping(ICIM)methods.The results showed that 5 significant markers for incidence of disease(ID)and disease Index(DI)were found using one-way ANOVA analysis.Meanwhile,5 QTLs were detected by ICIM method.Among them,2 QTLs were related with ID and 3 were related with DI.These results add to our understanding of the inheritance of resistance to CMV and provide information for marker assisted breeding for improvement of resistance to CMV in tobacco.