Src激酶抑制剂PP2对人舌鳞状细胞癌Tca8113细胞迁移和侵袭能力的影响

Effect of Src kinase inhibitor PP2 on migration and invasion of human oral tongue squamous cell carcinoma Tca8113 cells

目的探讨Src激酶抑制剂PP2对人舌鳞状细胞癌Tca8113细胞迁移和侵袭能力的影响。方法将对数生长期的Tca8113细胞分为0、1、5、10、20μmol·L^(-1)PP2组,分别加入0、1、5、10、20μmol·L^(-1)Src激酶抑制剂PP2进行培养,采用划痕实验和Transwell迁移实验检测Tca8113细胞的迁移能力,Transwell侵袭实验检测Tca8113细胞的体外侵袭能力。结果 PP2作用24 h时,0、1、5、10、20μmol·L^(-1)PP2组细胞迁移率随PP2浓度的增加逐渐降低(P<0.05),穿膜细胞数随PP2浓度的增加逐渐减少(P<0.05),细胞侵袭个数随PP2浓度的增加逐渐减少(P<0.05)。结论 Src激酶抑制剂PP2可以抑制人舌鳞状细胞癌细胞Tca8113的迁移和侵袭能力。

Objective To explore the effect of Src kinase inhibitor PP2 on the migration invasion of human oral tongue squamous cell carcinoma Tca8113 cells.Methods Tca8113 cells in logarithmic phase were collected and divided into 0,1,5,10,20μmol·L-1 PP2 group.The cells in the 0,1,5,10,20μmol·L-1 PP2 group were treated with different concentrations of Src kinase inhibitor PP2(0,1,5,10,20μmol·L-1)respectively.The migration ability of Tca8113 cells was detected by the scratch test and Transwell assay.The invasion ability of Tca8113 cells was detected by Transwell chamber.Results At 24 h after intervented by PPA,the migration rate of Tca8113 cells decreased with the increase of PP2 concentration in the 0,1,5,10,20μmol·L-1 PP2 group(P<0.05).The number of transmembrane cells in the 0,1,5,10,20μmol·L-1 PP2 group reduced with the increase of PP2 concentration(P<0.05).The number of invasion cells in the 0,1,5,10,20μmol·L-1 PP2 group decreased with the increase of PP2 concentration(P<0.05).Conclusion Src kinase inhibitor PP2 can inhibit the invasion and migration of human oral tongue squamous cell carcinoma Tca8113 cells.