菠萝VOZ转录因子序列特征及其对非生物胁迫的响应

Sequence Characteristics and Expression Analysis of VOZ Transcription Factors in Pineapple under Abiotic Stress

为探究菠萝(Ananas comosus)维管植物锌指蛋白转录因子(VOZ)家族的序列特征及表达特征,该研究采用生物信息学的方法对菠萝VOZ转录因子家族进行序列分析,并通过qRT-PCR技术研究了NaCl、干旱和低温(4℃)等逆境处理对菠萝VOZ家族基因表达的影响。结果表明:(1)菠萝与拟南芥、水稻的VOZ转录因子家族均含有2个保守区域,且菠萝VOZ蛋白均为亲水酸性蛋白;蛋白质二级结构显示,菠萝VOZ蛋白主要结构元件为α-螺旋和无规则卷曲。(2)qRT-PCR分析显示,不同非生物胁迫下,AcoVOZ1和AcoVOZ2的表达量与对照差异显著,且NaCl、干旱、低温(4℃)、甘露醇、ABA、Eth胁迫下,AcoVOZ1和AcoVOZ2的表达量均呈显著下降趋势。研究推测,菠萝VOZ转录因子家族以负调控的方式参与菠萝抗高盐、干旱、低温(4℃)等非生物胁迫的应答反应,为进一步研究菠萝抗逆分子机制和菠萝抗逆性基因工程改良奠定了技术基础。

In order to investigate the sequence characteristics and expression of VOZ(vascular plant one-zinc finger protein)transcription factors in pineapple,we analyzed the sequences of VOZ transcription factors in pineapple were analyzed by bioinformatics software.qRT-PCR was performed to analyze the expression patterns of VOZ transcription factors in pineapple seedlings under the treatments of NaCl,drought,low temperature(4℃),mannitol,Eth,SA,ABA,H 2O 2 and PEG6000.The results showed that:(1)the VOZ transcription factors in pineapple,Arabidopsis thaliana and rice all contained two conserved regions,and protein analysis showed that the AcoVOZ was hydrophilic acid protein.Secondary structure prediction showed that the main structural elements of AcoVOZ protein were alpha-helix and random coil.(2)qRT-PCR analysis showed that the expression of AcoVOZ 1 and AcoVOZ 2 were significantly different from the control under different abiotic stress conditions.Among them,the expression levels of AcoVOZ 1 and AcoVOZ 2 showed a significant decrease under the treatments of NaCl,drought,low temperature(4℃),mannitol,ABA,and Eth.The results suggested that VOZ transcription factors in pineapple participate in the response process of pineapple under various abiotic stresses such as high-salt,drought and low temperature(4℃),which may be helpful for the further study on molecular mechanisms of pineapple stress resistance and genetic improvement of pineapple resistance.