IL-8的变化在慢阻肺患者血清及支气管肺泡灌洗液中的应用价值研究

Study on the Value of IL-8 in Serum and Bronchoalveolar Lavage Fluid in Patients with COPD

目的:研究并探讨IL-8表达水平变化在慢性阻塞性肺疾病患者血清及支气管肺泡灌洗液中的应用价值。方法:笔者所在医院2015年1月-2017年1月收治的55例慢性阻塞性肺疾病急性发作患者、40例慢性阻塞性肺疾病稳定期患者纳入研究,将其归为慢阻肺组,根据其病情发作情况将患者分为急性期组、稳定期组,另选取同期30例健康受检者设为对照组。所有受检者均空腹静脉血液标本采集、支气管肺泡灌洗检查,测定其血清IL-8表达水平、支气管肺泡灌洗液中IL-8表达水平,比较慢阻肺组与对照组、急性期组与稳定期组、入院第1天急性期组患者与入院第7天急性期组患者的血清及支气管肺泡灌洗液中IL-8表达水平,采用皮尔逊相关系数分析法,计算IL-8表达水平与慢性阻塞性肺疾病的相关性。结果:慢阻肺组患者的血清IL-8表达水平、支气管肺泡灌洗液中IL-8表达水平均明显高于对照组(P<0.05);急性期组患者的血清IL-8表达水平、支气管肺泡灌洗液中IL-8表达水平均明显高于稳定期组患者(P<0.05);入院第7天,急性期组患者的血清IL-8表达水平、支气管肺泡灌洗液中IL-8表达水平均低于入院第1天(P<0.05);经皮尔逊相关系数分析,血清和支气管肺泡灌洗液中的IL-8表达水平均与慢性阻塞性肺疾病密切相关,且呈正相关。结论:IL-8可能参与到慢性阻塞性肺疾病的发生、发展过程中,可对慢性阻塞性肺疾病气道炎症程度予以反映,可作为慢性阻塞性肺疾病早期诊断、病情评估的重要指标。

Objective:To study and explore the changes in IL-8 expression in patients with chronic obstructive pulmonary disease and the practical value of bronchoalveolar lavage fluid.Method:From January 2015 to January 2017,55 cases of chronic obstructive pulmonary disease in acute attack patients and 40 patients with stable chronic obstructive pulmonary disease patients in our hospital were selected as the COPD group,according to the onset of the disease,the patients were divided into the acute phase group and the stable group,the others 30 cases were healthy subjects served as the control group.All subjects were collected fasting venous blood specimens,bronchoalveolar lavage.Determination of the serum expression levels of IL-8 and bronchoalveolar lavage fluid in the expression of IL-8,COPD group and control group,acute group and stable group,the first day of admission in patients with acute group seventh days after admission the patient’s blood group in acute stage and bronchoalveolar lavage fluid in the expression of IL-8 were compared,using the Pearson correlation coefficient analysis,calculated between the expression level of IL-8 and chronic obstructive pulmonary disease.Result:The expression level IL-8 in serum and bronchoalveolar lavage of COPD patients were significantly higher than those of the control group(P<0.05),the expression level of IL-8 in serum and bronchoalveolar lavage of acute patients were significantly higher than those in stable group(P<0.05).Seventh days after admission,the expression level of IL-8 in serum and bronchoalveolar lavage in acute patients was lower than that of the first day(P<0.05),correlation analysis of serum and bronchoalveolar lavage fluid in the expression level of IL-8 by Pearson was closely associated with chronic obstructive pulmonary disease,and were positively correlated.Conclusion:IL-8 may be involved in chronic obstructive pulmonary disease in the course of development,it can reflect the degree of airway inflammation in chronic obstructive pulmonary disease,and can be used as an important indicator for the early diagnosis of chronic obstructive pulmonary disease and the assessment of the condition of the disease. 【Key