微小RNA-143/145基因多态性与冠心病危险因素及严重程度的相关性

Associations of the MicroRNA-143/145 Polymorphisms with Cardiovascular Risk Factors and the Severity of Coronary Heart Disease

目的探讨中国汉族人群miR-143/145基因rs353292和rs4705343多态性与冠心病(CHD)患者心血管病危险因素及严重程度的相关性。方法应用聚合酶链式反应-限制性片段长度多态性法对380例CHD患者和163例对照者进行rs353292和rs4705343多态性分型。在对照组及CHD组中,比较不同基因型者之间生理生化指标的差异。在CHD组中,比较不同基因型者之间心肌梗死(MI)的发生率。结果在对照组中,rs353292位点TT基因型者血清三酰甘油(F=3.00,P=0.05)和血糖(F=9.91,P<0.001)水平显著高于C等位基因携带者,rs4705343位点TT基因型者患高血压的比例(χ~2=6.35,P=0.04)显著高于C等位基因携带者,差异均有统计学意义。在CHD组中,rs353292位点TT基因型患者血清超敏C反应蛋白(hs-CRP)(F=8.43,P<0.001)水平显著高于C等位基因携带者,同时TT基因型患者MI的发生率(χ~2=5.29,P=0.02)显著高于C等位基因携带者,差异均有统计学意义。结论 rs353292多态性T等位基因与CHD患者血清hs-CRP水平相关,可能通过miR-143/145调控hs-CRP基因表达,从而影响MI的发生和发展。rs4705343多态性与冠心病风险及严重程度不相关。

Objective To explore the possible effects of the microRNA(miR)-143/145 polymorphisms on cardiovascular risk factors and the severity of coronary heart disease(CHD)in Chinese Han people.Methods Polymerase chain reaction-restriction fragment length polymorphism analysis was employed to identify the genotypes of the rs353292 and rs4705343 polymorphisms for 380 patients with CHD and 163 CHD-free controls.The physiological and biochemical parameters between the genotypes were compared in the CHD patients and in controls,and the incidence of myocardial infarction(MI)was also compared between the genotypes in the CHD patients.Results The subjects with the rs353292 TT genotype had higher serum levels of triglycerides(F=3.00,P=0.05)and glucose(F=9.91,P<0.001)than the C carriers,and the subjects with the rs4705343 TT genotype had significantly higher prevalence of hypertension(χ2=6.35,P=0.04)than the C carriers in the control group.The patients with the rs353292 TT genotype had significantly higher serum levels of hypersensitive C-reactive protein(hs-CRP)(F=8.43,P<0.001)than the C carriers in the CHD group,and the frequency of MI was significantly higher in the patients with the rs353292 TT genotype than that in the C carrier patients(χ2=5.29,P=0.02).Conclusions The T allele of the rs353292 polymorphism is associated with serum hs-CRP levels in CHD patients,and it may affect the occurrence and development of MI by up-regulation of CRP gene through miR-143/145.The rs4705343 polymorphism is not related to the risk and severity of CHD.