金露梅提取物对α-淀粉酶、α-葡萄糖苷酶和醛糖还原酶的抑制作用

Inhibition of Potentilla fruticosa extracts on α-amylase,α-glucosidase and aldose reductase

通过α-淀粉酶抑制剂、α-葡萄糖苷酶抑制剂和醛糖还原酶抑制剂体外筛选模型,研究金露梅水提物及95%甲醇提取石油醚萃取部位、乙酸乙酯萃取部位和水层部位对α-淀粉酶、α-葡萄糖苷酶和醛糖还原酶的抑制活性作用;通过糖耐量实验测定金露梅提取物对小鼠餐后血糖的影响。结果显示,金露梅水提物及95%甲醇提取石油醚萃取部位、乙酸乙酯萃取部位对α-淀粉酶的半数抑制浓度(IC_(50))分别为0.432、1.193、0._(50)7 mg/mL;对α-葡萄糖苷酶的IC_(50)分别为0.164、0.768、0.466 mg/mL;对醛糖还原酶的IC_(50)分别为0.742、2.158、1.098 mg/mL。结果表明,金露梅水提物和金露梅乙酸乙酯萃取部位对α-淀粉酶、α-葡萄糖苷酶和醛糖还原酶的抑制活性较高。同时,金露梅水提物与乙酸乙酯萃取部位均能显著降低小鼠的餐后血糖水平。

This study measured the in vitro inhibitory effects ofα-amylase(AM),α-glycosidase(AG)and aldose reductase(AR)extraction from Potentilla fruticosa in three solvents:water extract(WE)and 95%methanol extraction of petroleum ether part(MEP),95%methanol extraction of ethyl acetate part(MEE)and 95%methanol extraction of water part(MEW)throughα-amylase inhibitors(AMI),α-glycosidase inhibitors(AGI)and aldose reductase inhibitors(ARI)activity screening models.In vivo effects of different solvents from Potentilla fruticosa on impaired glucose tolerance of mice were also measured.Among them,WE,MEP and MEE exhibited against AMI activity with IC 50 values of 0.432,1.193 and 0.507 mg/mL,respectively.Three solvents against AGI activity with IC 50 values of 0.164,0.768 and 0.466 mg/mL,respectively.Three solvents against ARI activity with IC 50 values of 0.742,2.158 and 1.098 mg/mL,respectively.The study suggests that Potentilla fruticosa in water extract and 95%methanol extraction of ethyl acetate part demonstrated a stronger inhibitory effect on AM,AG and AR.Meanwhile,Potentilla fruticosa in water extract and 95%methanol extraction of ethyl acetate part can be significantly decreased the postprandial blood glucose in mice.