不同环境对快速老化雄性小鼠抑郁与焦虑情绪的影响

Effects of different environments on depression and anxiety in male SAMP8,a model of accelerated senescence

目的观察不同环境对阿尔茨海默病模型SAMP8快速老化雄性小鼠抑郁、焦虑情绪的影响。方法雄性3月龄SAMP8小鼠30只,称量体质量后随机分为3组,每组各10只。3组小鼠分别在标准环境、丰富环境和贫瘠环境中饲养8周,每周称量各组小鼠体质量并记录其生活状态,8周后采用自主活动和高架十字迷宫实验检测其行为学变化。结果 丰富环境中生活的SAMP8鼠群生活更为和谐,动物间活动和互动现象较多;贫瘠环境中生活的小鼠存在咬伤、死亡现象。8周末3组间体质量差异无统计学意义(P>0.05)。对于小鼠的自主活动次数和开放臂停留时间,丰富环境组与标准环境组之间无统计学差异(P>0.05);贫瘠环境组与标准环境组之间差异有统计学意义(P<0.01)。结论 丰富环境使SAMP8雄性小鼠生活活跃、鼠间交流增多,但尚不能认为其对焦虑、抑郁情绪产生影响。贫瘠环境使SAMP8雄性小鼠活动力下降,焦虑、抑郁症状加重。

Objective To examine the effects of different environments on depression and anxiety in male SAMP8,a model of accelerated senescence.Methods Thirty 3-month-old male SAMP8 mice were randomly divided into three groups(n=10 in each group).Mice were assigned to standard environment,enriched environment and impoverished environment for 8 weeks,respectively.The body mass and general status were evaluated weekly.Behavioral changes were assessed by using autonomic activities and elevated-plus maze test at 8 weeks.Results The SAMP8 mice in enriched environment had more harmonious life,more activities and interactions among animals.In contrast,the mice in impoverished environment suffered from bites and deaths.There was no significant difference in body mass among the three groups(P>0.05).As for the number of activities and the open arm retention time,the differences were significant between the impoverished environment group and the standard environment group(P<0.01)but not significant between the enriched environment group and the standard environment group(P>0.05).Conclusions The enriched environment can make the male SAMP8 mice more active in life and more interaction.But enriched environment does not have an effect on anxiety and depression.The impoverished environment can reduce the activity of male SAMP8 mice,resulting in being prone to anxiety and depression.