安全高效红曲米工程化生产技术开发

Development of engineering production technology for safe and high-potency red kojic rice

为了实现高色价低橘霉素红曲米的工程化生产,通过单因素试验和正交试验讨论了培养基p H值、培养时间、培养基装量、培养温度、接种量和养花温度对红曲米色价和橘霉素含量的影响,然后进行了3 T发酵罐制种试验、中试生产试验和工程化生产试验。结果表明,最佳培养基p H值为6.5、培养时间为8 d、培养基装量为500 g/浅盘、培养温度为32℃、接种量为9%、养花温度为40℃。3 T发酵罐制种试验结果表明,在发酵30 h左右,菌丝丰富,含量达到30 g/L以上,种子液黏稠、呈深红色,p H 5.0~5.5。在中试生产试验和工程化生产试验中,红曲米的产率都达到了40%以上,色价均在5 000 U/g左右,橘霉素含量都≤42μg/kg。

In order to realize the engineering production of red kojic rice with high color value and low citrinin content,the effects of culture pH,time,medium loading volume,culture temperature,inoculum and germination temperature on color value and citrinin content were explored by single factor and orthogonal tests,and then the seed production tests of 3 T fermenter,pilot production tests and engineering production tests were carried out.The results showed that the optimal culture conditions were medium pH value 6.5,culture time 8 d,medium loading volume 500 g/plate,culture temperature 32℃,inoculum 9%,germination temperature 40℃.The results of seed production tests of 3 T fermenter indicated that the fresh mycelial concentration reached above 30 g/L about fermentation 30 h,the seed liquid was dark red and viscous,the hypha was abundant and the pH value was 5.0-5.5.In the pilot production tests and the engineering production tests,the red kojic rice yield was about 40%,the color value was about 5 000 U/g,and the citrinin content was≤42μg/kg.