枯草芽孢杆菌STO-12脂肽类物质抑菌活性及其特性分析

Antifungal Activities and Characterization of Lipopeptides Produced by Bacillus subtilis STO-12

为了探索植物病害生物防治的新途径,对枯草芽孢杆菌(Bacillus subtilis)STO-12的脂肽类物质的抑菌活性和特性进行分析,采用滤纸片法、排油试验、乳化试验和表面张力测试检测脂肽类物质的抑菌活性和特性。研究结果表明,枣缩果病菌(Alternaria tenuissima)、杨树炭疽病菌(Colletotrichum gloeosporioides)和杨树腐烂病菌(Cytospora chrysosperma)的生长受到脂肽类物质的强烈抑制,最小抑菌浓度(MIC)分别为0.02,0.04和0.02 mg·disc-1;分别在高温处理、强酸强碱处理、蛋白酶处理、紫外线处理及室温保存条件下,STO-12的脂肽类物质仍能保持其抑菌活性的稳定;另外,通过3种不同的检测试验,发现STO-12的脂肽类物质还具有较好的表面活性剂的特性。综上所述,研究表明枯草芽孢杆菌STO-12分泌的脂肽类物质具有高效的抗真菌活性,较好的抑菌活性稳定性和较强的表面活性的特性,作为生防制剂和生物表面活性剂,在工业和田间应用中具有较大的应用潜力。

In order to explore new pathways for biocontrol of plant diseases,the antifungal activity and characterization of the lipopeptides produced by Bacillus subtilis STO-12 have been investigated.Filtering paper method,oil displacement test,emulsification activity test,and surface tension measurements were used to determine the antifungal activity and characterization of the lipopeptides.The results indicated that Alternaria tenuissima,Colletotrichum gloeosporioides,and Cytospora chrysosperma were strong inhibited by the lipopeptides,the minimum inhibitory concentrations(MICs)were revealed 0.02,0.04,and 0.02 mg·disc-1.Even after extreme temperature,pH,and protease treatments,UV radiation,and prolonged storage at room temperature,the antifungal activity of the lipopeptides remained stable.Moreover,the powerful biosurfactant properties of the lipopeptides had been found,as assessed by three different methods.This study demonstrates that antifungal lipopeptides secreted by B.subtilis STO-12 possess strong antifungal activity,good stability,and powerful biosurfactant properties.They may be useful as biocontrol agents and biosurfactants and have potential in industrial and field applications.