可降解木质纤维素的双菌共生菌丝球的制备

Study on Immobilization Method of Two-species Mycelial Pellet for Degradation of Lignocellulose

以共固定化菌丝球的产酶能力作为指标,将木质素降解菌G-13和纤维素降解菌X10-1-2进行共固定,探讨了X10-1-2的接入时间、接种量、pH值、摇床的转速和温度对菌丝成球和产木质纤维素酶的影响。结果表明,当G-13与X10-1-2同时接入、纤维素降解菌的接种量为20 mL(100mL培养基),培养基的pH值为5、摇床转速为160 r/min、温度为28℃时,锰过氧化物酶、漆酶、木质素过氧化物酶、纤维素酶和半纤维素酶的酶活达到最高,分别为31.246 U/L、16.048 U/L、157.528U/L、197.063 U/L和215.81 U/L。X10-1-2与G-13在共固定过程中,具有互补的性能,在产酶的过程中发挥了协同的作用,促进了木质素降解酶的分泌。所获得的双菌共生菌丝球的球体为白色,表面光滑,大小均匀,具有一定的机械强度,能够满足实践应用的需求。

In our research,enzyme production ability of immobilization mycelial pellet was used as an index,the immobilization mycelial pellet was immobilized with lignin-degrading fungus G-13 and cellulose-degrading bacteria X10-1-2.The influence of five factors(addition time,inoculation amount of cellulose-degrading bacteria,pH,temperature and rotational speed)to Mycelium pellet,ligninase production and cellulase production were studied.The results indicated that X10-1-2 and G-13 were added simultaneously and the inoculation amount was 20%,pH was 5,culture temperature was 28℃,rotational speed was 160 r/min,the manganese(MnP);laccase(Lac);lignin peroxidase(Lip);cellulose;hemicellulose were 31.246 U/L;16.048 U/L;157.528 U/L;197.063 U/L;215.81 U/L,respectively.X10-1-2 and G-13 have the complementary advantage on the immobilization process,develope the synergy effects and promote the production of ligninase.The immobilization mycelial pellet was white,smooth surface,uniform size and good elasticity,which can satisfy practical needs.