摘要
背景:胰岛移植是一种比较有效的糖尿病治疗方法,运用生物医学工程技术将生物材料引入胰岛移植,可保持胰岛活力,提高移植效率。目的:将培养的聚乙醇酸-胰岛移植物移植到糖尿病受体大鼠肌肉内,观察聚乙醇酸-胰岛移植物治疗糖尿病的效果。方法:取Wistar大鼠18只,采用腹腔注射链脲菌素方法建立糖尿病模型,建模成功后即刻随机分3组干预,对照组腿部肌肉注射生理盐水,实验1组腿部肌肉注射异种胰岛细胞,实验2组腿部埋植聚乙醇酸-异种胰岛细胞移植物。移植后3,7,14,21,30 d,检测各组大鼠血糖及胰岛素水平;移植后14,30 d,取实验组移植物标本,进行扫描电镜观察。结果与结论:(1)实验1组、实验2组移植后3,7 d的血糖值明显低于对照组(P<0.05),实验2组移植后14,21,30 d的血糖值低于实验1组(P<0.05);(2)实验1组、实验2组移植后3,7 d的胰岛素水平明显高于对照组(P<0.05),实验2组移植后不同时间点的胰岛素水平明显高于实验1组(P<0.05);(3)扫描电镜显示,移植后14 d,实验组可见较多形态良好的胰岛细胞仍黏附于聚乙醇酸纤维支架上,胰岛细胞间可见呈细纤维交织成网状的细胞外基质形成,在胰岛细胞周围可见少量红细胞;随着埋植时间的延长,聚乙醇酸纤维支架开始降解,纤维支架逐渐断裂,长的纤维降解为短的纤维,30 d后最终支架层层脱落成碎片;(4)结果表明,聚乙醇酸-胰岛细胞移植能有效降低糖尿病大鼠的血糖水平,并提高血清胰岛素水平。
BACKGROUND:Islet transplantation is an effective method for diabetes treatment.Using biomedical engineering technology to introduce biomaterials into islet transplantation can maintain the vitality of islet and improve the efficiency of transplantation.OBJECTIVE:To transplant the cultured polyglycolic acid(PGA)-islet into the muscle of diabetic recipient rats and to observe the effect of PGA-islet in the treatment of diabetes mellitus.METHODS:A diabetic model was established in 18 Wistar rats by the method of intraperitoneal injection of streptozotocin,and the model rats were randomly assigned into three groups.In the control group,normal saline was injected into the muscle of the rat leg.In the experimental group 1,allogeneic islet cells were intramuscularly injected into the rat leg.In the experimental group 2,PGA-allogeneic islet cell graft was intramuscularly injected into the rat leg.At 3,7,14,21 and 30 days after transplantation,blood glucose and serum insulin levels were detected in each rat.At 14 and 30 days after transplantation,graft specimens were taken from the experimental groups and observed by scanning electron microscopy.RESULTS AND CONCLUSION:(1)At 3 and 7 days after transplantation,the blood glucose levels in the two experimental groups were significantly lower than that in the control group(P<0.05),while the serum insulin levels in the two experimental groups were significantly higher than that in the control group(P<0.05).Compared with the experimental group 1,the blood glucose level in the experimental group 2 was significantly lower at 14,21 and 30 days after transplantation,while the serum insulin level in the experimental group 2 was significantly higher at each time point after transplantation(P<0.05).Under the scanning electron microscope,in the experimental groups,plenty of well-formed islet cells were adhered to the PGA fiber scaffold at 14 days after transplantation;a visible network of extracellular matrix with fine fibers formed in islet cells;and a small number of red blood cells were visible around the islet cells.With the extension of embedded time,PGA fiber scaffolds began to degrade and crack.Long fibers degraded into short fibers,and after 30 days,the scaffold layer was broken into pieces finally.All these findings indicate that the transplantation of PGA-islet can effectively reduce the blood glucose level and increase the serum insulin level in diabetic rats.
作者
谢万均
魏争
宋纯
Xie Wan-jun;Wei Zheng;Song Chun(Dalian Friendship Hospital,Dalian 116021,Liaoning Province,China;Key Laboratory of Cell Transplantation,Ministry of Health,Department of General Surgery,First Affiliated Hospital of Harbin Medical University,Harbin 150000,Heilongjiang Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2018年第30期4829-4834,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金资助项目(30570931)~~