银离子注入氧化钛涂层植入物的抗菌性能

Antibacterial properties of titanium oxide coating with silver ions

背景:纳米银颗粒是一种十分具有潜力的内植物抗菌涂层材料;离子注入法可使银离子与基材牢固结合,同时有效控制银离子的注入量;氧化钛涂层具有良好的生物相容性,是制备内固定材料的良好选择。目前尚缺乏采用银离子注入法制备的氧化钛涂层内植物的体内抗菌性研究,其抗菌强度与离子注入量的关系也未明确。目的:观察银离子注入氧化钛涂层内植物在新西兰大白兔体内的抗菌性能。方法:将40只新西兰大白兔模型随机分成4组,钛棒组、单纯氧化钛组、低银氧化钛组、高银氧化钛组。建立股骨远端骨髓感染模型,各组分别植入相应的内植物。通过血液学、影像学及菌落计数法评价动物感染程度,以进一步探究银离子注入氧化钛涂层内植物的体内抗菌能力。结果与结论:(1)造模后各组动物体温均有不同程度升高,体质量下降,至14-21 d后逐渐恢复;(2)造模后7,14,21,28 d,钛棒组和单纯氧化钛组白细胞计数和C-反应蛋白水平均显著高于低银和高银氧化钛组(P<0.05);造模后7 d单纯氧化钛组中性粒细胞百分比均显著高于低银和高银氧化钛组(P<0.05);造模后14,21,28 d,钛棒组和单纯氧化钛组中性粒细胞百分比显著高于低银和高银氧化钛组(P<0.01);(3)造模后14和28 d,低银和高银氧化钛组的Norden评分显著低于钛棒组和单纯氧化钛组(P<0.05);(4)细菌培养菌落计数显示,钛棒组和单纯氧化钛组菌落计数显著高于低银和高银氧化钛组(P<0.01);低银氧化钛组显著高于高银氧化钛组(P<0.05);(5)结果提示,银离子注入氧化钛涂层内植物具有抗菌性,其抗菌性与银离子含量密切相关,即银离子含量增大其涂层抗菌性更强,其应用有可能为内植物相关感染提供有效的解决途径。

BACKGROUND:Silver nanoparticle is a potential coating material with resistance ability to infection.Using the ion implantation method,we can make silver nanoparticles tightly connect with the base material,and meanwhile effectively control the content of silver nanoparticles.Titanium oxide coating has good biocompatibility,which is a good choice for the preparation of internal fixation materials.But the in vivo antibacterial activity of titanium oxide coating with silver ions is rarely reported,and the relationship between the content of silver nanoparticles and the antibacterial activity is still unknown.OBJECTIVE:To investigate the antibacterial of silver plasma immersion ion implantation implants in vivo.METHODS:Forty New Zealand white rabbits were randomized into four groups,and animal models of bone marrow infection of the distal femur were made in all the rabbits.Model rabbits were subjected to the implantation of titanium rob in control group,implantation of titanium oxide-coated implants in experimental group,implantation of implants covered with titanium oxide coating with low concentration of silver ions in low concentration group,and implantation of implants covered with titanium oxide coating with high concentration of silver ions in high concentration group.We assessed the antibacterial activities of the implants by blood test,radiographic observation and bacteriological methods thereafter.RESULTS AND CONCLUSION:(1)After modeling,the body temperature in all the animals increased to different extents,and the body weight decreased,and gradually recovered at 14-21 days after modeling.(2)At 7,14,21,28 days after modeling,white blood cell count and C-reactive protein level in the titanium rod group and titanium oxide group were significantly higher than those in the low and high concentration groups(P<0.05).At 7 days after modeling,the percentage of neutrophils in the titanium oxide group was significantly higher than that in the low and high concentration groups(P<0.05).At 14,21,28 days after modeling,the percentage of neutrophils in the titanium rod group and the titanium oxide group was significantly higher than that in the low and high concentration groups(P<0.01).(3)At 14 and 28 days after modeling,the Norden scores in the low and high concentration groups were significantly lower than those in the titanium rod group and the titanium oxide group(P<0.05).(4)The number of bacterial colonies in the titanium rod group and titanium oxide groups was significantly higher than that in the low and high concentration groups(P<0.01),while the bacterial colony count in the low concentration group was significantly higher than that in the high concentration group(P<0.01).Findings from the present study reveal that the titanium oxide-coated implant with silver ions is an antibacterial implant,and its antibacterial property is closely related to the concentration of silver ions,that is,a higher concentration of silver ions indicates stronger antibacterial property.Therefore,the use of titanium oxide coating with silver ions may provide an effective solution for implant-related infections.