Vc二步发酵后期补充伴生菌对发酵产古龙酸的影响

Effect of Supplementation of Associated Bacteria on Fermentation of Gulongic Acid in the Late Stage of Vc Two-Step Fermentation

研究了Vc二步发酵后期补充伴生菌对产酸菌产古龙酸的影响。结果表明,在Vc二步发酵后期,产酸量和产酸速率低时,加入适量伴生菌(巨大芽孢杆菌)培养液或伴生活性物质对产酸菌(普通生酮基古龙酸菌)产酸有明显促进作用,提高了古龙酸产量并缩短了发酵周期。研究结果对查明Vc二步混菌发酵过程及机理、优化发酵后期工艺条件提供了理论依据。

The effects of adding companion strains to fermentation broth at later fermentation on 2-keto-L-gulonic acid production were studied.Results showed that adding suitable amount of companion strain or its active substances at later fermentation could stimulate Ketogulonicigenium vulgare growth and 2-KLG production,and shorten fermentation period.The results of our study provided theoretical basis for illuminating fermentation mechanism and optimizing fermentation process of the two-step mixed fermentation of Vc.