番茄溃疡病的接种方法及微生物防治菌剂筛选

Inoculation Method of Tomato Bacterial Canker and Screening of Microbial Control Agent

为筛选操作简单、显症时间短、真实反映番茄溃疡病发病程度的病原菌接种方法,比较了剪顶法、茎上针刺法、浸根法和拌土法4种不同接种方法对番茄溃疡病发病程度的影响。结果表明,剪顶法接种效果最好,发病率达100%,病情指数在90以上,操作简单,适用于番茄溃疡病菌的致病性测定和药效评价试验。通过室内盆栽试验,比较23种微生物菌剂对番茄溃疡病的防治效果,筛选出安全、高效的微生物菌剂3种,分别为芽孢IVF 018号、芽孢IVF 021号和芽孢IVF 020号,防效均高于98%。

In order to screen a simple,rapid and effective method for inoculation of tomato bacterial canker,this paper compared the effects of 4 different inoculation methods including top leaf shearing,needle penetrating,root soaking and bacterial-soil mixing methods on tomato canker occurance degree.The results showed that top leaf shearing was the best inoculation method.Its incidence rate was 100%,and disease index was over 90.This method is simple to operate,applicable for evaluating the pathogenicity of bacterial and fungicide control effect to tomato bacterial canker.Through pot experiment in greenhouse,this paper compared the control effects of 23 microbial agents for tomato bacteria canker and screened 3 safe and highly efficient microbial agents,naming bacillus IVF 018,bacillus IVF 021 and bacillus IVF020.Their control effects were all over 98%.