豌豆肽对环磷酰胺致免疫抑制小鼠免疫功能的影响

Effects of pea peptide on immune function in immunosuppressed mice induced by cyclophosphamide

研究豌豆肽对免疫抑制小鼠的免疫调节作用。腹腔注射环磷酰胺(cyclophosphamide,CTX)建立小鼠免疫抑制模型,并设置空白对照组,观察不同剂量豌豆肽[低剂量组0.4 g/(kg·bw)、中剂量组0.8 g/(kg·bw)、高剂量组1.6 g/(kg·bw)]干预15 d后对小鼠体重、免疫脏器重量及形态、白细胞计数、脾脏T淋巴细胞亚群、免疫球蛋白含量、细胞因子水平、骨髓有核细胞计数和DNA含量的影响。模型组小鼠脾脏和胸腺镜下形态学结构紊乱,外周血白细胞计数、CD3^+、CD4^+、CD8^+T淋巴细胞百分比、免疫球蛋白Ig G和Ig M含量、骨髓有核细胞浓度和DNA含量均显著低于对照组(p<0.01),细胞因子IFN-γ浓度明显低于对照组(p<0.05)。与模型组相比,豌豆肽组小鼠在以上指标有不同程度的改善。一定剂量的豌豆肽对CTX致免疫抑制小鼠的免疫功能具有一定的改善作用。

The aim of this study was to investigate the immuno-regulatory effects of pea peptides on immunosuppressive mice.Mice injected with cyclophosphamide(CTX)was the model group and normal saline was the control group.Different dosages of pea peptide(0.4 g/(kg·bw),0.8 g/(kg·bw),1.6 g/(kg·bw)were given to low,middle and high groups for 15 days.The body weight,spleen,and thymus weight of mice,morphologic variations of immune organs,white blood cell count,T lymphocyte subsets of spleen,immunoglobulin content,cytokine levels,bone marrow nucleated cell count and DNA content were tested.The morphological changes of spleen and thymus were observed under microscope in the model group.The number of white blood cell,proportion of CD 3+,CD 4+,CD 8+T lymphocyte,concentration of immunoglobulin IgG and IgM,and nucleated cell counts and DNA density in bone marrow were significantly lower than the control group(p<0.01).The concentration of IFN-γwas significantly lower than that of the control group(p<0.05).Compared with the model group,pea peptide groups showed different degrees of improvement in the above indicators.Pea peptide can improve the immune function of immunosuppressive mice induced by CTX.