多重荧光PCR方法检测食品中致病菌的实用研究

Multiple Real-time PCR Method for Pathogenic Detection in Food

目的:验证多重荧光PCR方法在食品中致病菌检测的实用性,并为标准制定提供参考。方法:以沙门氏菌、金黄色葡萄球菌、副溶血性弧菌、单核细胞增生李斯特氏菌为测试对象,设立阳性、阴性、空白样品对照,以GB4789.4-2016、GB 4789.7-2013、GB 4789.10-2016、GB 4789.30-2016为方法对照,设计引物和探针对样品进行检测。结果:多重荧光PCR方法的阳性、阴性、空白样品对照结果准确,检测结果与上述国标一致。结论:采用荧光PCR方法,可大幅缩短食品中致病菌的检测时间,且操作简便,具有一定的实用价值。

Applicability of multiple real-time PCR method for pathogenic detection in food was verified for the edition of relative standards.The testing pathogens contain Salmonella,Staphylococcus aureus,Listeria monocytogenes,and Vibrio parahemolyticus.The primers and probes were designed from 16S rRNA of these pathogens.The sample controls contained positive,negative,and blank controls.The method controls included GB 4789.4-2016,GB 4789.7-2013,GB 4789.10-2016,and GB 4789.30-2016.The reports showed that these sample controls were correct and the detection results were similar with these method controls.The real-time PCR method could shorten time,reduce operation,and possess applicability.