补肾强脊颗粒联合塞来昔布对强直性脊柱炎成纤维细胞抗骨化作用及BMP/Smad信号通路的影响

The effect of Bushen Qiangji particles combined with celecoxib on the antiossification of AS fibroblast and BMP/Smad signaling pathway

目的:探讨补肾强脊颗粒联合塞来昔布对强直性脊柱炎(AS)成纤维细胞抗骨化作用及BMP/Smad信号通路的影响。方法:选取疼痛科收治的AS患者100例,采用随机数字表法分为两组。对照组口服塞来昔布胶囊;观察组口服塞来昔布胶囊加用补肾强脊颗粒。结果:治疗后两组临床疗效各指标均较前显著改善(P<0.05),且观察组在BASDAI积分(13.29±5.13)分、BADFI积分(21.48±6.47)分、疼痛积分(1.56±0.54)分、晨僵时间(18.57±7.93)分,均较对照组显著改善(P<0.05)。两组Schober试验、扩胸度、指地距均较前显著改善(P<0.05),且观察组在Schober试验(4.42±1.14)较对照组(3.84±1.21)显著改善(P<0.05)。观察组SPARCC评分(6.27±3.26)分,对照组(7.35±2.45)分均较前显著改善(P<0.05)。两组BMP受体水平均较前显著改善(P<0.05),且观察组BMP-Ⅱ受体水平(0.042±0.003)显著降低于对照组(0.087±0.004),(P<0.05)。治疗后两组Smad水平均较前显著改善(P<0.05),且观察组Smad1水平(0.806±0.048)、Smad5水平(0.413±0.078)显著降低于对照组(P<0.05)。结论:补肾强脊颗粒联合塞来昔布通过抑制成纤维细胞中BMP/Smad信号通路的活化来延缓AS患者向成骨型分化,发挥抗骨化作用。

Objective:To investigate the effect of Bushen Qiangji particles combined with celecoxib on the antiossification of AS fibroblast and BMP/Smad signaling pathway.Methods:Select our hospital 100 cases of the AS patients,using the random number table method,divided into two groups.Control group oral celecoxib capsules,observation group oral celecoxib capsules combined with Bushen Qiangji particles.Results:After treatment,the clinical efficacy of the two groups was significantly improved(P<0.05),and the observation group of BASDAI score(13.29±5.13),BADFI score(21.48±6.47),pain score(1.56±0.54),and morning stiffness time(18.57±7.93).Significantly improved compared with the control group(P<0.05).The two groups Schober test,chest expansion,finger-to-ground distance were significantly improved(P<0.05),and the observation group in the Schober test(4.42±1.14)compared with the control group(3.84±1.21)was significant improvement(P<0.05).SPARCC score(6.27±3.26)points in the observation group,(7.35±2.45)points in the control group were significantly improved(P<0.05).BMP receptor levels in the two groups were significantly improved than before(P<0.05),and the observation group BMP-II receptor level(0.042±0.003)was significantly lower than the control group(0.087±0.004),(P<0.05).After treatment,the two groups Smad levels were significantly improved(P<0.05),and observed the level of Smad1 in the group was(0.806±0.048)and the Smad5 level was(0.413±0.078),which was significantly lower in the control group(P<0.05).Conlusion:Bushen Qiangji particles combined with celecoxib can inhibit the activation of BMP/Smad signaling pathway in fibroblasts and delay the differentiation of AS patients into osteogenesis and play an anti-ossification role.