叶绿素对乳酸链球菌生长特性和代谢水平的影响

Effects of Chlorophyll on Growth Characteristics and Metabolic Level of Lactic streptococci

为探究叶绿素是否具有生物活性作用,帮助人们理解摄入绿色蔬菜的意义,以乳酸链球菌为研究对象,将其(1.5 m L)接种到20 m L液体厌氧培养管中,同时加入1 m L叶绿素粗提液,以不加叶绿素粗提液为空白对照,置于37℃的水浴摇床中(70 r/min)培养24 h,探究叶绿素对乳酸链球菌生长特性及代谢产物的影响。试验结果表明叶绿素能促进乳酸链球菌增殖,且0~2 h为有效增长期;同时和对照组相比,叶绿素显著增加乳酸链球菌代谢产物乳酸、柠檬酸、正戊酸水平(P<0.05),且丙酸水平微量上升,草酸水平显著降低(P<0.05),所以叶绿素具有一定的生物活性作用。

In this study,the Lactic streptococci was used as the research object to investigate the biological activity of chlorophyll and to help people to understand the meaning of intake of green vegetables.The Lactic streptococci(1.5 mL)was inoculated into 20 mL of liquid anaerobic tubes,while 1 mL of chlorophyll was added.The effects of chlorophyll on the growth characteristics and metabolic level of Lactic streptococci were studied in a 37℃water bath(70 r/min)for 24 h and the sample without chlorophyll was considered as a blank control.The results showed that chlorophyll could promote the proliferation of Lactic streptococci and 0-2 h was the effective growth period.Meanwhile,chlorophyll increased significantly the levels of lactic acid,citric acid and n-pentanoic acid compared to the control(P<0.05).The levels of propionic acid increased slightly and the levels of oxalic acid decreased obviously(P<0.05).Hence,chlorophyll had a certain biological activity.