摘要
以湖北产地厚朴为原料,对其蛋白质提取方法进行优化,以满足蛋白质电泳的要求。比较3种不同蛋白质提取方法的提取率,选取超声破碎仪提取法进一步优化,通过单因素试验和正交试验确定最佳条件为超声时间30 min、超声时间间隔6 s、超声功率250 W,得到的蛋白质浓度为930.2μg/m L,研究表明此法可为厚朴的蛋白质电泳指纹图谱研究奠定基础。
The protein extraction method in Magnolia officinalis from Hubei was optimized to meet the requirements of protein electrophoresis.By comparing the protein extraction yield of three different methods,ultrasonic extraction method was selected for further optimization.The optimum conditions were determined by single factor test and orthogonal test,which were as follows:the ultrasonic time 30 min,ultrasonic interval 6 s,ultrasonic power 250 W.Under this condition,the concentration of protein was 930.2μg/mL.The results showed that the method could lay a foundation for the study of protein fingerprint of Magnolia officinalis.
作者
翁德会
杨文婷
汤雅萍
范承涛
刘翠
程勋
WENG De-hui;YANG Wen-ting;TANG Ya-ping;FAN Cheng-tao;LIU Cui;CHENG Xun(College of Biology and Pharmaceutical,Wuhan Huaxia University of Technology,Wuhan 430223,Hubei,China;Environmental and Biological Engineering College,Wuhan Technology and Business University,Wuhan 430065,Hubei,China)
出处
《食品研究与开发》
CAS
北大核心
2018年第18期66-69,共4页
Food Research and Development
基金
湖北省大学生创新创业训练计划项目(201713242012)
湖北省教育厅科学研究计划指导性项目(B2017394)
关键词
厚朴
蛋白质提取
超声破碎提取法
Magnolia officinalis
protein extraction
ultrasonic breakage extraction