人鼻咽癌组织环状RNA差异表达分析

Analysis of differential expression of circular RNAs in human nasopharyngeal carcinoma

目的环状RNA(circRNA)在肿瘤发生发展中发挥重要作用,但其与鼻咽癌的关系鲜有报道。文中旨在检测circRNA在人鼻咽癌组织及慢性鼻咽黏膜炎组织的表达谱差异。方法应用高通量circRNA芯片技术,采集2016年11月至2017年3月在广东医科大学附属医院进行活检的鼻咽癌组织和鼻咽慢性黏膜炎组织标本各3份,分析circRNA表达谱差异。通过对原始数据进行预处理、归一化,筛选出差异表达的circRNA,并对其进行聚类分析,Gene Ontology(GO)分析及KEGG Pathway分析,分析差异circRNA的功能分类及相关通路。结果鼻咽癌组织与慢性鼻咽黏膜炎组织之间共筛选出差异表达circRNA 829个,其中鼻咽癌组织中表达上调者761个,下调者68个。差异表达circRNA主要与细胞周期、细胞增殖等生物学进程有关;主要参与p53信号通路,细胞周期信号通路和DNA复制信号通路等。结论差异表达的circRNA与鼻咽癌的发生发展密切相关。

Objective Circular RNA(CircRNA)plays an important role in the carcinogenesis and development of cancers.However,the relationship between circRNA and nasopharyngeal carcinoma(NPC)has been rarely reported.The aim of this study is to investigate the differentially expressed circRNAs in human NPC and chronic nasopharyngeal mucositis. Methods Three NPC specimens and three chronic nasopharyngeal mucositis specimens which were diagnosed by nasopharyngeal biopsy in the Affiliated Hospital of Guangdong Medical University from November 2016 to March 2017,were enrolled in the study.The circRNA expression profiles of those candidates were assayed by high-throughput human circRNA microarray.After pretreatment and homogenization of the original data,the circRNAs with differential expression were screened out and analyzed by hierarchical clustering.Moreover,Gene Ontology(GO)analysis and KEGG pathway analysis were performed to analyze the functional classification and related pathways. Results Compared with the chronic nasopharyngeal mucositis,there are a total of 829 differentially expressed circRNAs in NPC,among which 761 were found to be up-regulated and 68 were down-regulated.Those differentially expressed circRNAs were analyzed to be mainly related to cell cycle,cell proliferation and other biological processes;mainly involved in p53 signaling pathway,cell cycle and DNA replication signaling pathway. Conclusion Those differentially expressed circRNAs may be associated with the tumorigenesis and development of NPC.