宫颈鳞癌及腺癌组织HPV感染型别分布的对比研究

Comparative study on genotype distribution of HPV infection in the tissues of cervical squamous cell carcinoma and cervical adenocarcinoma

目的国内有关女性宫颈鳞癌和腺癌组织HPV感染型别对比研究的大样本研究报道较少。文中旨在探讨区域性女性宫颈鳞癌和腺癌组织中HPV感染基因型分布状况和分型的临床意义。方法回顾性分析1978年11月至2017年12月29家区域性医院病理组织学诊断为女性宫颈鳞癌(n=826)和宫颈腺癌(n=218)石蜡组织标本1044例。从其组织中抽提HPV DNA,使用基因芯片结合聚合酶链式反应技术对所有宫颈鳞癌和腺癌组织检测23种HPV,并对受检者HPV感染型别分布状况进行对比分析。结果 1044例女性宫颈鳞癌和腺癌患者HPV检出者901例,检出率为86.30%。其中宫颈鳞癌检出率为91.53%(756/826),以16、18、58、33、52、31型最为常见,16型检出率显著高于18型(56.84%vs 9.93%,P<0.05);宫颈腺癌检出率为66.51%(145/218),以16、18、31、33、52、58型最为常见,16型与18型检出率差异无统计学意义(35.29%vs32.35%,P>0.05)。结论女性宫颈鳞癌和腺癌组织HPV检出率不同,16、18、31、33、52、58型是宫颈鳞癌和腺癌最主要的基因型,宫颈鳞癌中16型的检出频率远高出18型;而宫颈腺癌中16型和18型两型检出频率非常接近。

Objective There are few large sample data reports of comparative study on genotype distribution of human papillomavirus(HPV)infection in the tissues of cervical squamous cell carcinoma and cervical adenocarcinoma in China.This study aimed to investigate the clinical value of genotype distribution of HPV infection in the tissues of cervical squamous cell carcinoma and cervical adenocarcinoma in regional(mainly in Jiangsu Province)patients.Methods We collected 1044 paraffin tissue specimens of female cervical squamous cell carcinoma(826 cases)and cervical adenocarcinoma(218 cases)in 29 hospitals from November 1978 to December 2017.HPV DNA was extracted from the tissues and through the combination of gene-chip and polymerase chain reaction technology,23 genotypes of HPV were detected in all the tissues of cervical squamous cell carcinoma and cervical adenocarcinoma,and comparative study was conducted on the genotype distribution of HPV infection. Results Among 1044 cases of cervical squamous cell carcinoma and cervical adenocarcinoma,901 were found with HPV and the detection rate was 86.30%.The detection rate of cervical squamous cell carcinoma was 91.53%(756/826),among which 16,18,58,33,52,31 types were the most common and the detection rate of 16 type was significantly higher than that of 18 types(56.84%vs 9.93%,P<0.05).The detection rate of cervical adenocarcinoma was 66.51%(145/218),among which 16,18,31,33,52,58 types were the most common,and the detection rates of 16 type and 18 type was of no significant(35.29%vs 32.35%,P>0.05). Conclusion The HPV detection rates are different in the regional female cervical squamous cell carcinoma and cervical adenocarcinoma tissues.16,18,31,33,52 and 58 types are the most common genotypes in cervical squamous cell carcinoma and cervical adenocarcinoma.The detection rate of 16 type is overly higher than that of 18 type in cervical squamous cell carcinoma,while the detection rates of 16 type and 18 type in cervical adenocarcinoma are very close.