Nafion/GOX/CS-g-C_3N_4/GCE的制备及其在葡萄糖生物传感器中的应用

Preparation of Nafion/GOX/CS-g-C_3N_4/GCE and Its Application in Glucose Biosensor

首先以三聚氰胺为原料制备出g-C_3N_4纳米材料;再将壳聚糖(CS)与g-C_3N_4混合并滴涂在玻碳电极表面,制得CS-g-C_3N_4/GCE电极;最后将葡萄糖氧化酶(GOX)滴涂并用Nafion膜将其固定在CS-g-C_3N_4/GCE电极表面,制得Nafion/GOX/CS-g-C_3N_4/GCE葡萄糖生物传感器,实现了葡萄糖氧化酶的直接电化学。采用透射电子显微镜(TEM)、X-射线衍射仪(XRD)、循环伏安法(CV)及差分脉冲伏安法(DPV)等手段对所制备的电极进行了表征和测试。结果表明:所制得的Nafion/GOX/CS-g-C_3N_4/GCE葡萄糖生物传感器的响应范围为0.1~8.0mmol·L-1,检出限为46.7μmol·L-1,Michaelis-Menten常数为0.42mmol·L-1。该生物传感器可以排除尿酸(UA)、抗坏血酸(AA)、多巴胺(DA)等的干扰,表现出优异的抗干扰性和选择性。此外,该生物传感器还表现出较好的重复性和稳定性,可应用于实际样品检测。

In this paper,g-C3N4 nanocomposite were prepared with tripolycyanamide as raw materials.And then chitosan(CS)and g-C3N4 was mixed and coated on the surface of the glassy carbon electrode to prepare the CS-g-C3N4/GCE electrode.Finally,the Nafion/GOX/CS-g-C3 N4/GCE electrode based on direct electrochemistry of glucose oxidase(GOX)was fabricated by immobilizing GOX on glassy carbon electrode(GCE)functionalized by CS-g-C3N4 nanocomposites.The electrode was characterized by transmission electron microscopy(TEM),X-ray diffractometer(XRD),cyclic voltammetry(CV)and differential pulse voltammetry(DPV).The results show that the Nafion/GOX/CS-g-C3 N4/GCE glucose biosensor had a response range of 0.1-8.0 mmol.L-1,a detection limit of 46.7 yniol.L-1,and a Michaelis-Menten constant of 0.42 mmol.L_1.The biosensor could eliminate the interference of UA,AA,DA,exhibiting the excellent selectivity and anti-interference performance.Moreover,the biosensor also showed pretty reproducibility and stability,and it can be applied to the detection of actual samples.