miR-200b对三阴性乳腺癌细胞MDA-MB-231的负性调控作用

miRNA-200 bsuppresses triple negative breast cancer cells in vitro

目的研究miRNA-200b对三阴性乳腺癌细胞增殖、侵袭能力的影响。方法定量PCR检测人乳腺癌细胞株MCF-7、BT-474、MDA-MB-453、SKBR3、MDA-MB-468、MDA-MB-231中miRNA-200a/b/c的表达情况,通过miR-200b抑制剂及拟似物分别抑制、促进miR-200b的表达后,观察癌细胞增殖能力、克隆形成及侵袭能力的变化。结果 miRNA-200家族在Luminal型乳腺癌细胞MCF-7、BT-474和Her-2表达型MDA-MB-453、SKBR3的表达量高于三阴性型MDA-MB-468、MDA-MB-231的表达。通过inhibitor、mimics分别抑制、促进MDA-MB-231细胞中miR-200b的表达后,结果显示miR-200b对肿瘤细胞的增殖有抑制作用(P<0.05),miRNA-200b组对肿瘤细胞的克隆形成有抑制作用(P<0.05),对癌细胞侵袭有抑制作用(P<0.05)。结论miRNA-200b对三阴性乳腺癌细胞MDA-MB-231具有负性调控作用,可以作为潜在的早期诊断及治疗靶点。

Objective To identify the regulation of miRNA-200b on proliferation of triple negative breast cancer cells.Methods The expressions of miRNA-200a/b/c in breast cancer cell lines MCF-7,BT-474,MDA-MB-453,SKBR3,MDA-MB-468,and MDA-MB-231 were detected by real-time PCR.MTT was used to detect MDA-MB-231 cell proliferation after miR-200b was downregulated and upregulated.The colony-forming efficiency of miR-200b was detected by colony forming assay.The transwell chamber was used to investigate the influence of miR-200b on invasion of MDA-MB-231 cells.Results miRNA-200a/b/c had relatively low expressions in triple negative breast cancer cell lines MDA-MB-468 and MDA-MB-231,but high expressions in luminal cells MCF-7,BT-474,and Her-2 positive cells MDA-MB-453,SKBR3.The upregulated miR-200b could suppress the proliferation,colony formation and cell invasion of MDA-MB-231 cells(P<0.05).Also the downregulated miR-200b promoted cell proliferation,colony forming and cell invasion(P<0.05).Conclusion miRNA-200b suppresses triple negative breast cancer cell MDA-MB-231,making it a potential biomarker and target for early diagnosis and therapy.