蒲公英萜醇对人乳腺癌细胞MCF-7增殖及凋亡的影响

Effect of Taraxerol on the Proliferation and Apoptosis of MCF-7 Human Breast Cancer Cells

目的:探讨蒲公英萜醇对人乳腺癌细胞MCF-7增殖、凋亡的影响及其可能的分子机制。方法:体外培养人乳腺癌细胞MCF-7,采用噻唑蓝比色法和平板克隆实验检测蒲公英萜醇对MCF-7细胞增殖的影响;Hoechst 33342荧光染色法检测细胞凋亡的形态变化;Western blot法检测凋亡相关蛋白的表达。结果:蒲公英萜醇对人乳腺癌细胞MCF-7有明显的增殖抑制作用(P<0.05);Hoechst染色结果显示,给药后细胞核内染色质凝集,核固缩,可见凋亡小体;Western blot结果显示,蒲公英萜醇可极显著提高Bax/Bcl-2的比值(P<0.01)以及上调活化型Caspase-3/9的表达(P<0.01),下调PARP的表达(P<0.01)。结论:蒲公英萜醇能够明显抑制人乳腺癌细胞MCF-7增殖,并通过线粒体途径诱导其凋亡。

Objective:To investigate the effect of taraxerol on the proliferation and apoptosis of breast cancer MCF-7 cells and to explore its possible mechanism of action.Methods:The effect of taraxerol on the proliferation of MCF-7 cells was detected by methylthiazolyldiphenyltetrazolium bromide assay and colony formation assay;cell apoptosis was tested by Hoechst 33342 fluorescence staining,and apoptosis-related protein expression was detected by Western blot.Results:Taraxerol significantly inhibited the proliferation of MCF-7 cells(P<0.05).Hoechst staining revealed a series of morphological changes in MCF-7 cells associated with apoptosis,and Western blot data indicated that taraxerol significantly increased the ratio of Bax/Bcl-2(P<0.01)and up-regulated cleaved caspase-3/9 expression(P<0.01),and down-regulated PARP expression(P<0.01).Conclusion:Taraxerol can inhibit the proliferation,and induce the apoptosis of MCF-7 cells via the mitochondrial signaling pathway.