玉米南方锈病抗病QTL鉴定和效应分析

Mapping and effectiveness analysis for resistance genes of southern corn rust in maize

以玉米抗病自交系P 178、感病自交系G 41构建的BC2F5群体为材料,通过遗传连锁图谱构建和表型鉴定,对玉米南方锈病抗性进行了QTL定位。结果表明,在玉米第2,5,6和10染色体上共鉴定出5个抗病位点,可以解释6.88%到45.31%的表型遗传变异。其中,位于第10染色体短臂上的位点q SCR10.01具有最大的效应值。进一步对qSCR10.01进行精细定位的结果表明,抗病基因位于标记UMC1380和C(10)3595071之间,标记间的物理距离为1.34 Mb。

This study was to use quantitative trait loci(QTL)technique to map the resistance genes of southern corn rust(SCR)through genetic linkage map construction and phenotypic identification.The model material BC2F5 population was developed from the cross P178×G41,in which the line P178 was resistant to SCR and G41 was susceptible to SCR,respectively.Combined with the construction of genetic map with 349 KASP markers,quantitative trait loci(QTL)for SCR score was analyzed.Five QTL were identified on chromosomes 2,5,6 and 10,respectively,representing 6.88%~45.31%phenotypic variation.Of the five QTL identified,the one on chromosome 10(qSCR10.01)has biggest effect on,which can explain 45.31%phenotypic variation.The results also showed that,by further narrowing down of major QTL qSCR10.01,the QTL region was limited in between of flanking markers UMC1380 and C(10)3595071,which was 1.34 Mb in size based on the B73 reference genome.