北极黄河站邻近海域浮游细菌多样性初步研究

Preliminary study on the diversity of planktonic bacteria in the adjacent sea area of the Arctic Yellow River Station

通过荧光染色、稀释培养、生理生化和16SrRNA基因鉴定等方法,研究了北极黄河站附近海域海水中浮游细菌的丰度、可培养浮游细菌形态学特征及多样性;采用高通量测序技术研究了黄河站附近采样站点(BJ2)的不可培养浮游细菌群落结构。可培养研究结果表明:(1)9个站点(BJ1-BJ9)水样平均含菌数为2.88×108个/L;(2)21株可培养浮游细菌中,仅AB08、AB09和AB17三株细菌为革兰氏阳性菌;(3)细菌主要包括放线菌纲(Actinobacteria),黄杆菌纲(Flavobacteriia)和γ-变形菌纲(Gammaproteobacteria),其中假单胞菌属(Pseudomonas)为优势菌(占71%)。高通量测序结果显示:在得到的1 467个不可培养浮游细菌OTU中,变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes)为优势菌群,所占比例分别为49.71%和41.61%,还有少量的浮霉菌门(Planctomycetes)和疣微菌门(Verrucomicrobia);其中变形菌门中以α-变形菌纲(α-Proteobacteria)为主要类群。

This study investigated the abundance,morphological characteristics,and diversity of cultivable planktonic bacteria in surface seawater collected from the Arctic Ocean using fluorescence enumeration,dilution culture techniques,physiological test,and 16S rRNA gene identification method.In addition,the community structure of uncultured bacterioplankton was analyzed using high-throughput technology.The cultivable planktonic bacterial results showed that(1)the average number of bacteria from BJ1–BJ9 was 2.88×108 cells/L;(2)AB08,AB09,and AB17 strains were Gram-positive;(3)the marine cultivable bacteria were primarily composed of Actinobacteria,Flavobacteriia,and Gammaproteobacteria,among which Pseudomonas was dominant(71%).The high-throughput sequencing technology resulted in 1467 OTUs,which were dominated by Proteobacteria and Bacteroidetes that accounted for 49.71%and 41.61%,respectively.They also included a small amount of Planctomycetes and Verrucomicrobia.Alphaproteobacteria was the predominant class in Proteobacteria.