高产铁载体产生菌的筛选及其在发酵绿茶中的初步应用

Isolation of Siderophore-Producing Microorginisms and Study on It's Application in Fermentation of Green Tea

铁载体在植物营养、诱导植物抗性及人体保健方面具有广泛的应用前景。利用CAS双层平板法从69株苏铁根际微生物中筛选出8株高产铁载体的芽孢杆菌,经鉴定发现8株菌所产铁载体主要为儿茶酚盐型。综合考察8株芽孢杆菌铁载体的产量和发酵后绿茶感官特征,最终筛选出一株两者俱佳的菌,通过分子和生理生化鉴定、菌落形态比较初步鉴定为特基拉芽孢杆菌。最后对特基拉芽孢杆菌液态发酵产茶的条件进行优化。在40℃,接种0.5 mL/g,发酵5 d时利用该菌发酵绿茶得到的茶叶口感、香气、色泽最佳。

Siderophores have broad applications in plant nutrition,induction of plant resistance and human health-care.CAS double-layer plateswere employed in order to select siderophore-producing microorganisms.Eight high-yield siderophore-producing Bacillus bacteria strains were selected from the 69 microorganisms which were isolated from roots of cycad.And their siderophores were mainly phenolatescatecholates.Finally,a strain of high siderophore production was chosen,and it revealed to be Bacillus tequilensis by comparsion of 16s rRNA.Bacillus tequilensis produced the best fermented green tea at 40℃and incolubation volume of 0.5 mL/g after 5 d.