摘要
目的:通过比较淋巴细胞主动免疫治疗不明原因的复发性流产(URSA)前后血清免疫组学表达,评价淋巴细胞免疫治疗的临床意义。方法:选取54例封闭抗体阴性BA(-)的复发性流产患者为研究对象,观察治疗后的BA表达和妊娠情况,通过流式细胞术和免疫比浊法检测外周血T细胞亚群以及外周血免疫球蛋白的变化。结果:相对于治疗前,治疗后BA(-)组的患者中T细胞亚群CD_3^+、CD_8^+亚群数量增加,CD_4^+及CD16^+CD56^+亚群比例下降,外周血免疫球蛋白IgM和IgG表达下降,差异均存在统计学意义(P<0.05);IgA变化没有显著性差异(P>0.05);BA阳性(+)组患者的所有指标与治疗前相比均无统计学差异(P>0.05)。阳性BA(+)组妊娠成功率达91.4%,BA(-)组妊娠成功率为36.8%,两组妊娠成功率间差异存在统计学意义(P<0.05)。结论:淋巴细胞主动免疫治疗复发性流产效果显著,T细胞亚群的变化可能有利于妊娠,封闭抗体治疗值得临床推广。
Objective:To compare the serum immunohistochemistry before and after Lymphocyte active immunotherapy in unexplained recurrent spontaneous abortion(URSA)and normal pregnant,in order to evaluate the clinical significance of Lymphocyte immunotherapy.Methods:A total of 65 blocking antibody(BA)negative expression URSA patients were performed in our hospital.To observe the pregnancy progress,T-lymphocyte subsets and peripheral blood immunoglobulin changes after BA treatment.Results:Compared with before treatment,the number of CD 3+and CD 8+subpopulations of CD 4+and CD 16+CD 56+subsets decreased,and the expression of IgM and IgG in peripheral blood decreased in patients with BA-after treatment.Significance(P<0.05);there was no significant difference in IgA changes(P>0.05).There was no significant difference in all indexes of patients in the BA+group compared with treatment before(P>0.05).The success rate of pregnancy in the BA+group was 91.4%,and the success rate in the BA-group was 36.8%,and there was a statistically significant difference between the two groups(P<0.05).Conclusion:The change of T-lymphocyte subsets could be good for pregnancy.Lymphocyte active immunotherapy is significant in URSA,which worth clinical promotion.
作者
郝莉娜
Hao Lina(Department of Obstetrics and Gynecology, The First Hospital of Yulin City,Shaanxi Province(Yulin 719000))
出处
《陕西医学杂志》
CAS
2018年第10期1294-1296,共3页
Shaanxi Medical Journal