AM真菌对百合调节激素平衡与细胞渗透性以及改善耐盐性的研究

Research of Arbuscular Mycorrhizal Fungus on Adjusting Hormone and Osmotic Balance and Improving Salt Tolerance of Lilium brownii

该试验以东方百合(Lilium brownii)品种‘西伯利亚’和丛枝菌根(AM)真菌摩西斗管囊霉(Funneliformis mosseae)、变形球囊霉(Glomus versiforme)为材料,在温室盆栽条件下,设置NaCl胁迫(0、0.4%、0.8%和1.2%NaCl溶液处理)和接种AM真菌[接种摩西斗管囊霉、变形球囊霉、摩西斗管囊霉+变形球囊霉及未接种对照]双因素试验,分析各处理百合的激素平衡与细胞渗透性变化特征,以明确AM真菌提高百合耐盐性的效应,初步探索AM真菌增强百合耐盐性的作用机制。结果表明:(1)接种AM真菌能有效增加盐胁迫下百合植株的株高和生物量,显著提高百合耐盐系数,双接种处理的株高及地上、地下部分干重在1.2%NaCl胁迫下分别比未接种对照显著增加8.9%、14.5%和11.2%。(2)接种AM真菌能显著提高盐胁迫下百合植株叶片的矿质元素P、K、S含量,显著降低叶片内Na和Fe含量,双接种处理的P、K、S含量在1.2%NaCl胁迫下分别比对照提高10.9%、8.3%、13.7%,而其Na和Fe含量分别显著下降28.4%和66.4%。(3)接种AM真菌能显著提高盐胁迫下百合内源激素吲哚乙酸(IAA)和脱落酸(ABA)含量,双接种处理在1.2%NaCl胁迫下百合内源IAA、ABA含量分别是对照的1.2和1.5倍。(4)接种AM真菌能显著提高盐胁迫下百合可溶性蛋白含量,显著降低其脯氨酸含量,双接种处理在1.2%NaCl胁迫下比对照的升降幅度分别为69%和31%。(5)接种AM真菌能显著降低盐胁迫下百合叶片丙二醛和相对电导率,双接种处理在1.2%NaCl胁迫下比对照分别显著下降58.1%和9.0%。研究发现,AM真菌可以通过增强百合对养分的吸收、降低氧化胁迫造成的伤害、调节植物内源激素平衡状况与细胞渗透性来增强自身的耐盐能力,且双接种处理的效果优于单一接种。

Oriental lily species‘Siberian’and arbuscular mycorrhizal fungus Funneliformis mosseae and Glomus versiforme were used as materials in this experiment.The NaCl stress(0,0.4%,0.8%and 1.2%NaCl solution treatment)and inoculation with AM fungi[inoculation with F.mosseae,G.versiforme,F.mosseae+G.versiforme and non-inoculation treatment]were tested in two factors under greenhouse conditions.We analyzed the characteristics of hormone balance and cell permeability of lily under different treatments,in order to evaluate the effect of AM fungus on salt tolerance and explore the mechanism of AM fungus enhancing salt tolerance of lily.The results showed that:(1)AM fungus could effectively increase the plant height and biomass of lily plants under salt stress,and significantly improve the salt tolerance of lily.The double inoculation treatment resulted in the increase of plant height,dry weight above ground and underground,reaching 8.9%,14.5%,and 11.2%respectively as compared with the uninoculated control under 1.2%NaCl stress.(2)AM fungus can significantly increase the contents of mineral element P,K,S and reduce the contents of Na and Fe in leaves of lily plants under salt stress.Compared with the control,the contents of P,K and S in leaves of lily plants with double inoculation treatment were increased 10.9%,8.3%and 13.7%,while their Na and Fe contents decreased by 28.4%and 66.4%respectively.(3)AM fungus can significantly increase the contents of endogenous hormone indole-acetic acid and abscisic acid under salt stress.The double inoculation treatment was 1.2 times and 1.5 times higher than that in the control under 1.2%NaCl stress,respectively.(4)AM fungus can significantly increase the soluble protein content of lily and significantly decrease its proline content under salt stress.The increase and decrease amplitude of double inoculation treatment was 69%and 31%under the 1.2%NaCl stress.(5)AM fungus can significantly reduce the malondialdehyde content and relative conductivity of lily leaves under salt stress.The double inoculation treatment decreased by 58.1%and 9.0%under the stress of 1.2%NaCl,respectively.It can be seen that AM fungus can enhance the tolerance of lily under salt stress by enhancing the absorption of nutrients,reducing the damage caused by oxidative stress,regulating the balance of endogenous hormones and cell permeability,and the effect of double inoculation is better than that of single inoculation.