乳酸菌发酵废液制备酸化剂及其应用效果研究

Study on the preparation of acidifying agent from lactic acid bacteria fermented wastewater and its application effects

利用乳酸菌生产过程中的发酵废液制作酸化剂,以期将高浓度有机废液经济地、高效地转化为资源性产品。收集植物乳杆菌(ATCC 14917)发酵后产生的废液制成酸化剂,检测总糖、还原糖、粗蛋白、氨基酸及金属离子等指标,并按一定比例加入到仔猪饮水中,检测分析动物的生长性能、肠道菌群结构及免疫性能。结果表明:该发酵废液制成的酸化剂(p H3.0~3.5)主要成分如下:总糖含量2.2%,还原糖含量0.035%,粗蛋白含量1.13%,氨基酸含量1.09%。将酸化剂以0.5%的添加量加入仔猪的饮水系统中,饲喂周期35 d,与对照组相比,酸化剂组仔猪的平均日增重提高13.8%(P<0.05),料肉比降低18.1%(P<0.05),腹泻率降低22.9%(P<0.05);粪便中变形菌门菌群和螺旋菌门菌群显著降低,饲喂至第18 d时,乳杆菌属的相对丰度小幅增加;血清中HSP-40、Ig A、Ig M含量有不同程度提高。乳酸菌发酵废液制成的酸化剂,展现了良好的饲用价值,在环境保护和废液资源化利用方面应用前景广阔。

The fermented wastewater in the process of producing lactic acid bacteria was utilized to make the acidifying agent in order to convert the high concentration organic wastewater economically and efficiently into a resource product.The waste liquid produced by Lactobacillus plantarum(ATCC 14917)fermentation was collected,and the acidifying agent was prepared.The indexes,including total sugar,reducing sugar,crude protein,amino acid and metal ion,were detected.The acidifying agent added to drinking water at a certain proportion for piglets,whose growth,intestinal microflora and immune function of were analyzed.The results showed that the main ingredients in the acidifying agent(pH 3.0~3.5)were 2.2%total sugar,0.035%reducing sugar,1.13%crude protein and 1.09%amino acids.The acidifying agent was mixed into the piglets'drinking water system by 0.5%feeding for 35 d.Compared with the control group,in acidifying agent group,the daily weight gain of piglets increased significantly by 13.8%(P<0.05),the ratio of feed to meat and diarrhea rate decreased by 18.1%and 22.9%(P<0.05),respectively.The flora of proteobacteria and spirochaetae decreased significantly in the feces,and the relative abundance of lactobacillus.sp increased slightly after feeding for 18 d.The levels of HSP-40,IgA and IgM were significantly improved in the serum.The acidifying agent shows good feeding value made from the wastewater of lactic acid bacteria,and has a bright future in environmental protection and utilization of wastewater.