谷氨酰胺对高脂饮食诱导C57BL/6J小鼠肥胖和胰岛素抵抗的影响

Effect of L-glutamine on obesity and insulin resistance in high-fat die T-induced C57BL/6J mice

目的:探讨谷氨酰胺(L-glutamine,Gln)对高脂饮食(high-fat diet,HFD)诱导小鼠肥胖和胰岛素抵抗的影响。方法:60只雄性C57BL/6J小鼠随机分为正常对照(normal control,NC)组、HFD组、HFD+丙氨酸(Lalanine,Ala)组和HFD+Gln组,每组15只。每周记录小鼠体重,给药16周后禁食不禁水12 h测定空腹血糖(fasting blood glucose,FBG),处死后剖腹取附睾脂肪垫并称重。采用酶联免疫法检测小鼠胰岛素(insulin,INS)、瘦素(leptin,LEP)、脂联素(adiponectin,APN)和胰高血糖素样肽1(glucagon-like peptide-1,GLP-1)的水平,并计算胰岛素抵抗指数(insulin resistance index,IRI)和胰岛素敏感指数(insulin sensitivity index,ISI)。结果:与NC组比较,HFD组小鼠体重和附睾脂肪垫重量明显升高,FBG、INS、IRI和LEP水平均明显升高,ISI和APN水平明显降低(P<0.05);与HFD组比较,HFD+Gln组小鼠体重明显下降,FBG和LEP水平明显降低,IRI明显减小(P<0.05)。4组小鼠血清的GLP-1水平差异无统计学显著性。结论:谷氨酰胺减轻高脂饮食诱导的肥胖小鼠体重和胰岛素抵抗。

AIM:To explore the effect of L-glutamine(Gln)on obesity and insulin resistance in high-fat diet(HFD)-induced C57BL/6J mice.METHODS:Male C57BL/6J mice(n=60)were randomly divided into normal control(NC)group,HFD group,HFD+L-alanine(Ala)group and HFD+Gln group.Each group had 15 mice.The body weight of the mice was recorded weekly.Fasting blood glucose(FBG)of the mice was tested after 12-h fasting only with water-drinking at the end of the 16th week.The mice were sacrificed and epididymal fat pad was measured.The levels of insulin(INS),leptin(LEP),adiponectin(APN)and glucagon-like peptide-1(GLP-1)were measured by ELISA.The insulin resistance index(IRI)and insulin sensitivity index(ISI)were calculated.RESULTS:Compared with NC group,the body weight,epididymal fat pad weight,and the levels of FBG,INS,IRI and LEP increased significantly in HFD group(P<0.05),while the levels of ISI and APN decreased significantly(P<0.05).Compared with HFD group,the body weight,and the levels of FBG,IRI and LEP decreased significantly in HFD+Gln group(P<0.05),while the levels of ISI and APN increased significantly(P<0.05).No significant difference of serum GLP-1 levels the four groups was observed.CONCLUSION:L-glutamine reduces the body weight and attenuates the insulin resistance of HFD-induced mice.